| Genetic Diversity Analysis of PVY Resistant Potato Varieties （Clones） by RAPD Markers |
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| 引用本文： | DI Hong LIU Zhaojun CHEN Yili. Genetic Diversity Analysis of PVY Resistant Potato Varieties （Clones） by RAPD Markers[J]. 东北农业大学学报(英文版), 2006, 13(1): 16-21 |
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| 作者姓名： | DI Hong LIU Zhaojun CHEN Yili |
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| 作者单位： | College of Agriculture，Northeast Agricultural University，Harbin Heilongjiang 150030，PRC; Biotechnology Research Center，Heilongjiang Academy of Agricultural Sciences，Harbin Heilongjiang 150086，PRC |
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| 摘 要： | DNA fingerprints of 37 PVY resistant potato varieties (clones) were generated using PCR-based RAPD analysis. All 37 varieties were differentiated by banding patterns obtained from 17 primers that generated 164 polymorphisms. Similarity matrices were calculated with the simple matching coefficient. The genetic distance value among them was between 0.27 and 0.50, with average value being 0.35. The average link clustering algorithm was used to construct a dendrogram with Statistica. They were classified into three groups according to clustering results, and the results presented some geographic relationship. Dendrogram showed close genetic relationships between a number of varieties (clones) of similar pedigree. This study has shown that RAPD marker is a useful tool for potato variety identification, differentiation, and estimation of genetic relationships.
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| 关 键 词： | 马铃薯 RAPD PVY 遗传多样性 分子标记 |
| 文章编号： | 1006-8104（2006）-01-0016-06 |
| 收稿时间： | 2005-10-28 |
Genetic Diversity Analysis of PVY Resistant Potato Varieties (Clones) by RAPD Markers |
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| DI Hong,LIU Zhaojun,CHEN Yili. Genetic Diversity Analysis of PVY Resistant Potato Varieties (Clones) by RAPD Markers[J]. Journal of Northeast Agricultural University, 2006, 13(1): 16-21 |
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| Authors: | DI Hong LIU Zhaojun CHEN Yili |
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| Abstract: | DNA fingerprints of 37 PVY resistant potato varieties (clones) were generated using PCR-based RAPD analysis. All 37 varieties were differentiated by banding patterns obtained from 17 primers that generated 164 polymorphisms. Similarity matrices were calculated with the simple matching coefficient. The genetic distance value among them was between 0.27 and 0.50, with average value being 0.35. The average link clustering algorithm was used to construct a dendrogram with Statistica. They were classified into three groups according to clustering results, and the results presented some geographic relationship. Dendrogram showed close genetic relationships between a number of varieties (clones) of similar pedigree. This study has shown that RAPD marker is a useful tool for potato variety identification, differentiation, and estimation of genetic relationships. |
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| Keywords: | potato RAPD PVY genetic diversity |
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