Glycoprotein derived from the hot water extract of mint plant, Perilla frutescens britton |
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Authors: | Asada M Fukumori Y Inoue M Nakagomi K Sugie M Fujita Y Tomizuka N Yamazaki Y Oka S |
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Institution: | Cell Biology Laboratory, Biosignalling Department, National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki, Japan. |
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Abstract: | Glycoprotein showing inhibitory activity against mast cell degranulation and hyaluronidase activity was purified from the hot water extract of mint plant (Perilla frutescens Britton). The purified inhibitor gave a single band detected with Coomassie brilliant blue staining and periodic acid-Schiff staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The molecular mass was estimated to be 6.0 kDa on SDS-PAGE. The inhibitor did not become inactivated when boiled for 30 min or digested with trypsin, V8 protease, or proteinase K but was inactivated by NaIO(4) oxidation. The inhibitor prevented mast cell degranulation and hyaluronidase activity (IC(50) = 0.42 mg/mL) in a dose-dependent manner. The inhibitor also inhibited the protein kinase C activity. It is possible to purify and characterize a glycoprotein with putative pharmacological properties from mint plants. |
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