A simple method for the isolation of immunoglobulin (Y) from the eggs of immunized hens |
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Authors: | J Wallmann C Staak E Luge |
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Affiliation: | GTZ-BGA-Service-Labor im Institut für Veterin?rmedizin, Bundesgesundheitsamtes, Berlin. |
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Abstract: | Hens were vaccinated with Salmonella typhi-murium and with serum-IgG from a goat. Antibodies (IgY) were isolated from the eggs by ammonium sulphate precipitation. The concentration of egg antibodies was equal or superior to the concentration of serum antibodies of the hen. The longlasting antibody plateau of 9 and 28 weeks, respectively, provides evidence of the high yields of specific IgY which can be collected from a single immunized hen. |
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