| 花叶病大蒜的茎端组织脱毒培养 |
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| 引用本文： | 赵顺庆,李鹏飞,范怀忠,郭碧霞. 花叶病大蒜的茎端组织脱毒培养[J]. 华南农业大学学报, 1987, 0(4) |
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| 作者姓名： | 赵顺庆 李鹏飞 范怀忠 郭碧霞 |
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| 作者单位： | 华南农业大学植保系，华南农业大学园艺系，华南农业大学植保系，华南农业大学园艺系 中华人民共和国江门动植物检疫所 |
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| 摘 要： | 本文论述了用MS(1962)培养基添加吲哚乙酸(IAA)、细胞激动素(KT)、萘乙酸(NAA)、2,4—二氯苯氧乙酸(2,4—D)、6—苄基嘌呤(BA)等生长调节物质,对开平金山火蒜及广州硬尾骨蒜两品种茎端0.100～0.125mm长的分生组织进行培养,获得了无毒种蒜,并提出了适于此两种大蒜无毒苗培养的培养基配方、生长调节质物的组合和浓度、以及光温条件等一套方案;论证了2,4—D、NAA、BA、光照和温度对愈伤组织诱导、芽分化和球茎形成上的作用,并对大蒜不同组织部位的培养作了初步的探讨。
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| 关 键 词： | 大蒜花叶病 大蒜花叶病毒 茎端组织培养 脱毒组织培养 |
PRELIMINARY EXPERIMENT ON VIRUS-FREE GARLIC TISSUE CULTURE |
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| Zhao Shunqing Li Peng-fi Faan Hwei-chung Kuo Pihsia. PRELIMINARY EXPERIMENT ON VIRUS-FREE GARLIC TISSUE CULTURE[J]. JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY, 1987, 0(4) |
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| Authors: | Zhao Shunqing Li Peng-fi Faan Hwei-chung Kuo Pihsia |
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| Affiliation: | South China Agricultural University |
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| Abstract: | Two virus-free garlic cultivar bulbs were obtained after culturing 0.100 -0.125mm long shoot apices on the basal medium of Murashige and Skoog(1962) supplemented with different combinations and different concentration of IAA, 2,4-D, KT. NAA and BA. The effects of these growth regulators and the influences of light and temperature to the callus growth, organogenesis and bulb formtion had been compared and discussed, a set of culturing conditions and media with growth regulators were recommended. |
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| Keywords: | Garlic mosaic disease Garlic Mosaic Virus Tissue Culture Virus-free tissue culture |
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