中国明对虾FBA基因克隆及其在白斑综合征病毒感染中的表达及功能分析 cDNA Cloning of the FBA Gene in Fenneropenaeus chinensis and Its Expression and Functional Analysis After WSSV Infection期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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中国明对虾FBA基因克隆及其在白斑综合征病毒感染中的表达及功能分析

作者姓名：	史晓丽孟宪红孔杰栾生罗坤曹宝祥曹家旺陈宝龙

作者单位：	农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所青岛 266071；青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室青岛 266071,农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所青岛 266071；青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室青岛 266071,农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所青岛 266071；青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室青岛 266071,农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所青岛 266071；青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室青岛 266071,农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所青岛 266071；青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室青岛 266071,农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所青岛 266071；青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室青岛 266071,农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所青岛 266071；青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室青岛 266071,农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所青岛 266071；青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室青岛 266071

摘要：	醛缩酶(FBA)是糖酵解和糖异生中的关键酶,参与多种生物过程。本研究采用RACE技术,克隆获得中国明对虾(Fenneropenaeus chinensis)FBA基因(Fc FBA)的全长c DNA序列,并对其进行生物信息学分析。结果显示,中国明对虾Fc FBA基因的c DNA全长为2496 bp,其中,ORF长1098 bp,5~′UTR长79 bp,3~′UTR长1319 bp。完整的阅读框编码365个氨基酸,分子量为39.8 k Da,预测的理论等电点为6.6。同源性及系统进化分析表明,Fc FBA与节肢动物的FBA聚为一类,与卤虫(Artemia franciscana)、家蚕(Bombyx mori)、沙漠蝗(Schistocerca gregaria)的相似度分别是86%、79%和78%。荧光定量PCR结果显示,Fc FBA在肌肉中的相对表达量最高,肝胰腺中最低。WSSV感染后,该基因在鳃、肝胰腺和肌肉中呈现出不同的时空表达特点。ds RNA干扰24 h以后,抑制效率达到最大。与PBS对照组相比,Fc FBA干扰组(ds RNA组)加快了对虾染病后的死亡速度。本研究表明,Fc FBA基因可能参与了中国明对虾生物胁迫的应答反应。
关键词：	中国明对虾 FBA基因基因克隆组织表达基因干扰
收稿时间：	2017/2/20 0:00:00
修稿时间：	2017/3/21 0:00:00
cDNA Cloning of the FBA Gene in Fenneropenaeus chinensis and Its Expression and Functional Analysis After WSSV Infection

Authors:	SHI Xiaoli MENG Xianhong KONG Jie LUAN Sheng LUO Kun CAO Baoxiang CAO Jiawang and CHEN Baolong

Institution:	Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071 and Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071

Abstract:

Keywords:	Fenneropenaeus chinensis Fructose-1 6-bisphosphate aldolase gene Cloning Expression RNA interference
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