| 尼帕病毒G和F蛋白单克隆抗体的制备与鉴定 |
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| 引用本文： | 苏华,温志远,吴超,王喜军,胡森,陈伟业,葛金英,步志高.尼帕病毒G和F蛋白单克隆抗体的制备与鉴定[J].中国预防兽医学报,2011,33(11):898-901. |
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| 作者姓名： | 苏华 温志远 吴超 王喜军 胡森 陈伟业 葛金英 步志高 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室 农业部兽医公共卫生重点开放实验室,黑龙江哈尔滨,150001 |
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| 基金项目： | 公益性行业(农业)科研专项项目 |
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| 摘 要： | 为制备抗尼帕病毒(NiV)G和F蛋白的单克隆抗体(MAb),本研究以表达NiV G和F蛋白的真核重组表达质粒pCAGG-NiVG和pCAGG-NiVF分别免疫BALB/c小鼠,采用常规技术制备杂交瘤细胞；以表达G和F蛋白的重组牛痘病毒(rWR-NiVG、rWR-NiVF)分别感染BHK细胞,通过间接免疫荧光(IFA)筛...
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| 关 键 词： | 尼帕病毒 G和F蛋白 细胞融合 单克隆抗体 |
Preparation and identification of the monoclonal antibodies against G or F protein of Nipah virus |
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| SU Hua,WEN Zhi-yuan,WU Chao,WANG Xi-jun,HU Sen,CHEN Wei-ye,GE Jin-ying,BU Zhi-gao.Preparation and identification of the monoclonal antibodies against G or F protein of Nipah virus[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(11):898-901. |
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| Authors: | SU Hua WEN Zhi-yuan WU Chao WANG Xi-jun HU Sen CHEN Wei-ye GE Jin-ying BU Zhi-gao |
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| Institution: | SU Hua,WEN Zhi-yuan,WU Chao,WANG Xi-jun,HU Sen,CHEN Wei-ye,GE Jin-ying,BU Zhi-gao(Key Laboratory of Veterinary Public Health of Ministry of Agricultural,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | To prepare the monoclonal antibody(MAb) against G or F protein of Nipah virus(NiV),hybridoma cell lines were produced by fusinig SP2/0 cells with spleen cells from mouse immunized with pCAGG-NiVG or pCAGG-NiVF expressing G or F protein of NiV,respectively.Two hybridoma cell lines,designed G3E9 and F2G1 were indentified by indirect immunofluorescence assay,which were stablely secreting MAbs against G or F protein.The two MAbs were IgM subtype with kappa light chain.Both MAbs were able to reduce syncytium for... |
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| Keywords: | Nipah virus G and F proteins syncytium monoclonal antibodies |
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