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| J亚型禽白血病病毒的拯救与鉴定 | |
| 引用本文： | 王超,缪华先,谢宝婵,张伟伟,吴润,刘光清. J亚型禽白血病病毒的拯救与鉴定[J]. 中国预防兽医学报, 2011, 33(11). DOI: 10.3969/j.issn.1008-0589.2011.11.02 |
| 作者姓名： | 王超 缪华先 谢宝婵 张伟伟 吴润 刘光清 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃兰州730070;中国农业科学院上海兽医研究所,上海200241 2. 惠州出入境检验检疫局,广东惠州,516001 3. 中国农业科学院上海兽医研究所,上海,200241 4. 甘肃农业大学动物医学院,甘肃兰州,730070 |
| 基金项目： | 公益性农业科研专项项目，863计划项目，中央级公益性科研院所基本科研业务费专项项目 |
| 摘 要： | 为鉴定含J亚群禽白血病病毒(ALV-J)全长cDNA分子克隆(pBlALV)的感染性,首先将pBlALV转染CEF细胞,拯救出病毒(rALV),然后连续传代3次,对拯救病毒进行增殖培养.分别利用RT-PCR、western blot、IFA等方法,对拯救病毒进行鉴定,并应用实时荧光定量PCR方法对拯救病毒的复制动力学进行了测定.研究结果证明,本研究成功拯救出了具有感染性的rALV-J,为研究禽白血病的致病机理和探讨新的防制措施等提供了良好的ALV的反向遗传操作技术平台. |
| 关 键 词： | J亚群禽白血病病毒 ALV-J 感染性克隆 |
Rescue and identification of the infectious clone of subgroup J avian leucosis virus |
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| WANG Chao,MIAO Hua-xian,XIE Bao-chan,ZHANG Wei-wei,WU Run,LIU Guang-qing. Rescue and identification of the infectious clone of subgroup J avian leucosis virus[J]. Chinese Journal of Preventive Veterinary Medicine, 2011, 33(11). DOI: 10.3969/j.issn.1008-0589.2011.11.02 | |
| Authors: | WANG Chao MIAO Hua-xian XIE Bao-chan ZHANG Wei-wei WU Run LIU Guang-qing |
| Affiliation: | WANG Chao1,2,MIAO Hua-xian3,XIE Bao-chan3,ZHANG Wei-wei2,WU Run1,LIU Guang-qing2(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China,2.Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,3.Huizhou Entry-exit Inspection and Quarantine Bureau,Huizhou 516001,China) |
| Abstract: | To confirm the infectivity of the full-length cDNA clone(pBlALV) of subgroup J avian leucosis virus(ALV-J) HPRS103 strain,pBlALV was transfected into chicken embryo fibroblast(CEF) cells and the ALV was rescued.The rescued ALV(rALV-J) was identified by RT-PCR,western blot and indirect immunofluorescence assay,respectively.Besides,the real-time PCR was applied to evaluate the replication dynamic of the rescued virus.The results showed that the rALV-J was able to well replicate in CEF.This study provides an u... |
| Keywords: | subgroup J avian leucosis virus ALV-J infectious cDNA clone |
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