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| 氟喹诺酮类药物多残留间接竞争ELISA检测方法的建立 | |
| 引用本文： | 姜金庆,杨雪峰,王自良,常新耀,王三虎,刘兴友.氟喹诺酮类药物多残留间接竞争ELISA检测方法的建立[J].中国预防兽医学报,2011,33(11). |
| 作者姓名： | 姜金庆 杨雪峰 王自良 常新耀 王三虎 刘兴友 |
| 作者单位： | 1. 河南科技学院动物科学学院,河南新乡453003;动物疫病和残留物防控河南省高校工程技术研究中心,河南新乡453003 2. 河南科技学院动物科学学院,河南新乡,453003 3. 动物疫病和残留物防控河南省高校工程技术研究中心,河南新乡,453003 |
| 基金项目： | 国家十一五科技支撑计划重大项目，河南省教育厅科技攻关项目 |
| 摘 要： | 为建立同时检测多种氟喹诺酮药物的免疫学分析方法,本研究以碳二亚胺(EDC)二步法合成环丙沙星人工抗原,免疫新西兰大白兔制备多克隆抗体,建立氟喹诺酮类药物多残留检测方法.标准曲线在PBS中的线性检测范围为0.2 ng/mL～376 ng/mL,半数抑制浓度(IC50)为8 ng/mL,检测限(LOD)为0.1 ng/mL;抗体对恩诺沙星、诺氟沙星和培氟沙星均能特异性识别,IC50值分别为7.3 ng/mL、10.6 ng/mL、和13.2 ng/mL;标准品稀释液中NaOH和甲醇的最高容忍度分别为10％和30％；牛奶基质中添加5 ng/mL、20 ng/mL和50 ng/mL的标准品,环丙沙星、恩诺沙星、诺氟沙星和培氟沙星的回收率分别为93％～108％、96％～110％、92.5％～104％和93％～102％. |
| 关 键 词： | 环丙沙星 多克隆抗体 间接竞争ELISA 氟喹诺酮药物 多残留检测 |
Development of indirect competitive ELISA for detection of fluoroquinolone residues |
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| JIANG Jin-qing,YANG Xue-feng,WANG Zi-liang,CHANG Xin-yao,WAMG San-hu,LIU Xing-you.Development of indirect competitive ELISA for detection of fluoroquinolone residues[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(11). | |
| Authors: | JIANG Jin-qing YANG Xue-feng WANG Zi-liang CHANG Xin-yao WAMG San-hu LIU Xing-you |
| Institution: | JIANG Jin-qing1,2,YANG Xue-feng1,WANG Zi-liang2,CHANG Xin-yao1,WAMG San-hu2,LIU Xing-you2(1.College of Animal Science,Henan Institute of Science and Technology,Xinxiang 453003,China,2.Henan Higher Education Engineering Technology Research Center for Animal Diseases Control and Residues Supervison,China) |
| Abstract: | To develop a multidetermination immunoassay for fluoroquinolone(FQs) residues,an indirect competitive ELISA was developed with the polyclonal antibody against ciprofloxacin(CPFX pAb) produced from rabbits which immunized with FQs conjugated BSA.This method was sensitive and had a linear detection range from 0.2 ng/mL to 376 ng/mL(R2=0.943),with IC50 and LOD values of 8 ng/mL and 0.1 ng/mL.The antibody exhibited high sensitivity to FQs,and the IC50 values to enrofloxacin,norfloxacin and pefloxacin were 7.3 n... |
| Keywords: | ciprofloxacin polyclonal antibody indirect competitive ELISA fluoroquinolone multiresidue determination |
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