| 不同继代培养次数对苹果八棱海棠离体培养和遗传转化的影响 |
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| 引用本文： | 李淑艳,乔玉山,渠慎春,佟兆国,章镇. 不同继代培养次数对苹果八棱海棠离体培养和遗传转化的影响[J]. 果树学报, 2008, 25(6): 916-919 |
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| 作者姓名： | 李淑艳 乔玉山 渠慎春 佟兆国 章镇 |
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| 作者单位： | 南京农业大学园艺学院,南京210095 |
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| 基金项目： | 2008年度国家高技术研究发展计划(863计划) |
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| 摘 要： | 以苹果八棱海棠离体新梢和幼叶为外植体,研究不同继代次数对其离体繁殖、再生和遗传转化的影响。结果表明,随着继代培养次数的增加,继代10次和48次的八棱海棠离体增殖倍数、生根能力、不定芽的再生频率、每外植体再生芽数和GUS基因瞬时表达率均显著高于继代5次的;继代10次的增殖倍数和GUS阳性率明显多于继代48次的,其它指标相互间差异不显著;说明继代10次的芽苗较适合用于八棱海棠离体培养的外殖体材料,其幼叶较适于遗传转化研究。
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| 关 键 词： | 八棱海棠 继代培养次数 增殖 生根 不定芽再生 GUS瞬时表达率 |
Effects of different subculture times on isolated culture and genetic trans-formation of Malus micromalus |
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| LI Shu-yan,QIAO Yu-shan,QU Shen-chun,TONG Zhao-guo,ZHANG Zhen. Effects of different subculture times on isolated culture and genetic trans-formation of Malus micromalus[J]. Journal of Fruit Science, 2008, 25(6): 916-919 |
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| Authors: | LI Shu-yan QIAO Yu-shan QU Shen-chun TONG Zhao-guo ZHANG Zhen |
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| Affiliation: | LI Shu-yan,QIAO Yu-shan,QU Shen-chun,TONG Zhao-guo,ZHANG Zhen(College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095 China) |
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| Abstract: | The Malus micromalus shoot explants and young leaves in vitro were used as materials for studying the effects of dif-ferent subculture times on isolated culture and genetic transformation.The results showed that along with the increasing num-ber of subculture times,the isolated culture and transformation ability of the shoot explants subcultured in vitro for 10 and 48 times were higher than that of 5 times in shoot multiplication,rooting,adventitious bud regeneration rate and GUS transient expression from l... |
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| Keywords: | Malus micromalus Subculture times Shoot multiplication Rooting Adventitious bud regeneration GUS tran-sient expression |
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