大孔树脂分离纯化鼠曲草中黄酮类化合物研究 |
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引用本文: | 陆英,李洁,邱文敏,汤玉其,周兴凤,刘仲华. 大孔树脂分离纯化鼠曲草中黄酮类化合物研究[J]. 湖南农业大学学报(自然科学版), 2009, 35(5) |
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作者姓名: | 陆英 李洁 邱文敏 汤玉其 周兴凤 刘仲华 |
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作者单位: | 湖南农业大学,天然产物研究中心,湖南,长沙,410128;湖南农业大学,园艺园林学院,湖南,长沙,410128 |
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基金项目: | 湖南省科学技术厅项目 |
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摘 要: | 考察7种大孔吸附树脂对鼠曲草中黄酮类化合物的吸附分离性能,确定大孔树脂分离鼠曲草中黄酮类化合物的最佳工艺条件.结果表明,树脂对鼠曲草黄酮有良好的吸附分离性能,其最佳工艺条件为:ADS-21质量浓度1.28~1.78 mg/mL;pH 3.0;鼠曲草原料液以4 BV/h的流速上柱吸附后,再用8倍树脂体积的30%乙醇以3 BV/h的流速解吸,解吸率为95.5%;湿树脂的饱和吸附量为49.57 mg/mL;纯化产品中黄酮含量为82.23%.
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关 键 词: | 鼠曲草 黄酮 大孔吸附树脂 分离纯化 |
Separation and purification for flavones from Gnaphlium affine D. Don by macroporous resin |
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Abstract: | In order to set up a technique for adsorbing and separating flavones from Gnaphlium affine D. Don with macroporous adsorption resin, 7 types of resin were investigated. The results showed that ADS-21 resin was the best for adsorbing and separating flavones in the following technological conditions: the concentration of sample of extract, 1.28~1.78 mg/mL; pH3.0; the flow rate of fluid 4 BV/h, the eluting regent, 30%ethanol, 8 folds of the volume of the resin, elution rate 3 BV/h, desorbing rate was 95.5%; the maximum adsorbing capacity of ADS-21 resin for flavones was 49.57 mg/mL; the content of flavons in the purified products was 82.23%. |
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Keywords: | Gnaphlium affine D. Don flavones macroporous adsorption resin separation and purification |
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