| 杀虫基因高效植物表达载体的构建 |
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| 引用本文： | 邓智年,魏源文,黄诚梅,潘有强,吕维莉,李杨瑞.杀虫基因高效植物表达载体的构建[J].广西农业科学,2010,41(9):886-888. |
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| 作者姓名： | 邓智年 魏源文 黄诚梅 潘有强 吕维莉 李杨瑞 |
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| 作者单位： | 广西作物遗传改良生物技术重点开放实验室,南宁,530007 |
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| 基金项目： | 国家科技支撑计划课题项目，广西科技基础条件平台建设项目，广西科学研究与技术开发计划项目，广西科学基金项目，广西科学基金项目应用基础研究专项项目，广西农业科学院科技发展基金项目 |
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| 摘 要： | 以质粒pCAMBIA1200、pBI121、pMMAR和pC1300MAR为基础,通过酶切连接,构建植物中间载体pC2MARHyg;再利用pAVAc质粒、载体pGA1611和中间载体pC2MARHyg,构建含有Ubi-1启动子、顺向重复MAR和杀虫基因AVAc的高效植物表达载体pC2MAR-AVAc,然后转化农杆菌EHA105,经筛选鉴定获得阳性克隆,为进一步开展植物抗虫基因工程研究奠定基础。
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| 关 键 词： | 杀虫基因 植物表达载体 MAR序列 |
Construction of insect-resistant gene plant expression vector |
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| DENG Zhi-nian,WEI Yuan-wen,HUANG Cheng-mei,PAN You-qiang,Lü Wei-li,LI Yang-rui.Construction of insect-resistant gene plant expression vector[J].Guangxi Agricultural Sciences,2010,41(9):886-888. |
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| Authors: | DENG Zhi-nian WEI Yuan-wen HUANG Cheng-mei PAN You-qiang Lü Wei-li LI Yang-rui |
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| Institution: | DENG Zhi-nian,WEI Yuan-wen,HUANG Cheng-mei,PAN You-qiang,Lü Wei-li,LI Yang-rui*(Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007,China) |
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| Abstract: | Based on plasmids of pCAMBIA1200,pBI121,pMMAR and pC1300MAR,plant intermediate vector pC2MARHyg was constructed by digestion and ligation.Plant expression vector pC2MAR-AVAc containing Ubi-1 promoter,direct duplication MAR sequence and insect-resistant gene(AVAc),were constructed using pAVAc plasmid,pC2MARHyg vector and pGA1611 vector.The constructed vector was transformed to Agrobacterium EHA105.The newly constructed plant expression vector can be used in molecular breeding programs tailored for developing... |
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| Keywords: | insect-resistant gene plant expression vector MAR sequence |
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