Genetic diversity of Indonesian cauliflower cultivars and their relationships with hybrid cultivars grown in Australia |
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| Authors: | Ida A Astarini Julie A Plummer Rachel A Lancaster Guijun Yan |
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| Institution: | 1. School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia;2. Department of Agriculture, Western Australia, Bunbury District Office, PO Box 1231, Bunbury, WA 6231, Australia |
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| Abstract: | The objectives of this study were to investigate genetic variation and relationships between Indonesia-, Australian- and European-based cultivars and to evaluate variation within Indonesia cultivars as all cultivars are open-pollinated. Eight cauliflower cultivars collected from three production regions in Indonesia and four F1 hybrids cultivars grown in Australia were evaluated using RAPD and ISSR markers. DNA polymorphisms generated from 10 polymorphic RAPD primers were used to construct a dendogram using the unweighted pair-group method with arithmetic averages (UPGMA) and to generate a fingerprinting key. ISSR marker analysis using 14 primers were attempted but DNA polymorphisms could not be clearly identified. The RAPD technique indicated that variation occurred both within and between Indonesian cultivars. Comparison between Indonesian-, Australian- and European-based cultivars showed that Indonesian cultivars have unique genotypes and would be good sources of genes for future crop improvement. |
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| Keywords: | Brassica oleracea DNA fingerprinting DNA markers ISSR RAPD |
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