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Immunohistochemical localization of phospholipase D in strawberry (Fragaria ananassa Duch.) fruits
Authors:Haiying Yuan  Ligeng Chen  Gopinadhan Paliyath  Alan Sullivan  Dennis P Murr  Zuzana Novotna
Institution:1. Fruit Research Institute, Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, PR China;2. Department of Horticulture, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, PR China;3. Department of Plant Agriculture, University of Guelph, Guelph, Ont. NIG 2W1, Canada;4. Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technicka 3, 16628 Prague 6, Czech Republic
Abstract:Phospholipase D, the key enzyme involved in membrane phospholipid catabolism, was localized in strawberry (Fragaria ananassa Duch.) fruits during development using immunohistochemical techniques. Typical structural features such as the nucleus, cell wall, plasma membrane, mitochondria and vacuole were noticeable in cells of young strawberry fruits. Cytoplasmic structural features were less pronounced in fully grown and turning red fruits, except cell wall, which was considerably enlarged. Also, extensive microvesiculation from the plasma membrane was noticeable in ripening fruits. The number of visible gold particles indicative of phospholipase D increased during development. Phospholipase D was found to be associated with membranous structures and vacuole. Interestingly, gold-particles could also be observed in the cell wall space in ripening fruits suggesting that cytosolic contents may have leaked into the cell wall space as a result of membrane deterioration and loss of compartmentalization. The role of phospholipase D during development and ripening of strawberry, a non-climacteric fruit, is discussed.
Keywords:Fruit development  Membrane  Phospholipase D  Immunolocalization
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