| 中药防风对胃癌SGC-7901细胞生长及基因表达的研究 |
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| 引用本文： | 孙晓红,;李洪涛,;邵世和.中药防风对胃癌SGC-7901细胞生长及基因表达的研究[J].吉林林学院学报,2009(2):127-130. |
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| 作者姓名： | 孙晓红 ;李洪涛 ;邵世和 |
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| 作者单位： | [1]北华大学医学检验学院,吉林吉林132013; [2]北华大学基础医学院,吉林吉林132013; [3]江苏大学镇江医学院,江苏镇江212221 |
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| 基金项目： | 吉林市科技发展计划项目（2007023） |
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| 摘 要： | 目的研究中药防风对胃癌SGC-7901细胞生长及基因表达的影响．方法应用集落形成实验、MTT实验及电子显微镜技术观察了防风对SGC-7901细胞生长的影响；应用免疫组织化学染色技术研究防风对SGC-7901细胞基因表达的影响．结果防风对SGC-7901细胞的生长曲线、集落形成有明显的抑制作用，其抑制作用与防风的浓度和时间呈正相关关系，其IC50值为24g／mL．防风能使SGC-7901细胞p16和p21编码基因的蛋白表达上调，PCNA，C—Met编码基因的蛋白表达下调．结论防风能抑制SGC-7901细胞生长；防风可使SGC-7901细胞的p16，p21，PCNA，C—Met表达改变．这些结果为防风在临床上的进一步应用提供了理论依据．
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| 关 键 词： | 防风 SGC-7901细胞 p16 p21 PCNA C-Met |
On Cell Growth and Gene Expression of SGC-7901 Cells Induced by Fangfeng |
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| Institution: | SUN Xiao-hong , LI Hong-tao, SHAO Shi-he ( 1. Medical Inspection College of Beihua University, Jilin 132013, China; 2. Basic Medical College of Beihua University,Jilin 132013, China; 3. Zhenjiang Medical College of Jiangsu University, Zhenjiang 212221, China ) |
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| Abstract: | Objective To investigate the influence of Fangfeng on the cell growth and gene expression of SGC- 7901. Method The influence of Fangfeng on the cell growth of SGC-7901 was observed by using colony-forming test, MTT test and electronmicroscope;The influence of Fangfeng on gene expression of SGC-7901 was detected by using immunohistochemical assay. Results Cell growth curve and colony-forming test showed that the growth of SGC-7901 cells was significantly inhibited and the inhibition became stronger as the concentration and time increased. Its ICs0 is 24 g/mL. Fangfeng up-regulated p16 and p21 gene expression of SGC-7901, and down- regulated C-Met and PCNA gene expression. Conclusion Fangfeng inhibited the growth of SGC-7901 cells; Fangfeng altered the gene expression of p16, p21, PCNA and C-Met. These results provided a theoretical base for further clinical exploitation of Fangfeng. |
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| Keywords: | Fangfeng SGC-7901 cell p 16 p21 PC NA C-Met |
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