| 小麦品种绵麦37和绵麦367抗白粉病基因的FISH分析及分子检测 |
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| 引用本文： | 王洋洋,邱 玲,李 萌,汤述尧,符书兰,唐宗祥.小麦品种绵麦37和绵麦367抗白粉病基因的FISH分析及分子检测[J].麦类作物学报,2015,35(11):1502-1505. |
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| 作者姓名： | 王洋洋 邱 玲 李 萌 汤述尧 符书兰 唐宗祥 |
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| 作者单位： | （四川农业大学植物遗传育种省级重点实验室/四川农业大学农学院，成都温江 611130） |
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| 基金项目： | 国家自然科学基金项目（31470409） |
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| 摘 要： | 为了进一步研究和利用小麦品种绵麦37和绵麦367所携带的白粉病抗性基因,首先以OligopSc119.2-1(可检测小麦染色体结构变异)、Oligo-pTa535-1(可检测小麦染色体结构变异)和pDb12H(可检测簇毛麦染色体)为探针,对这两个小麦品种进行了荧光原位杂交(FISH)分析,然后利用与Pm21基因连锁的分子标记NAU/xibao15对这两个小麦品种进行分子检测。结果表明,绵麦37和绵麦367都含有1对6VS/6AL易位染色体,且均携带Pm21基因。
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| 关 键 词： | 小麦 白粉病 Pm21基因 FISH |
Molecular Detection and FISH Analysis of Powdery Mildew Resistance Gene in Wheat Cultivars Mianmai 37 and Mianmai 367 |
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| WANG Yangyang,QIU Ling,LI Meng,TANG Shuyao,FU Shulan,TANG Zongxiang.Molecular Detection and FISH Analysis of Powdery Mildew Resistance Gene in Wheat Cultivars Mianmai 37 and Mianmai 367[J].Journal of Triticeae Crops,2015,35(11):1502-1505. |
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| Authors: | WANG Yangyang QIU Ling LI Meng TANG Shuyao FU Shulan TANG Zongxiang |
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| Abstract: | For further utilization of powdery mildew resistant gene(s) in wheat cultivars Mianmai 37 and Mianmai 367, fluorescence in situ hybridization (FISH) by using probes Oligo-pSc119.2-1 (to detect structural variations of wheat chromosomes),Oligo-pTa535-1 (to detect structural variations of wheat chromosomes) and pDb12H (to detect chromosomes of Dasypyrum breviaristatum) was first used to analyze Mianmai 37 and Mianmai 367. Subsequently,molecular marker NAU/xibao15,which is linked toPm21 gene,was also used to analyze the two wheat cultivars. The results indicated that the two wheat cultivars contained a pair of 6VS/6AL translocation chromosomes andPm21 gene. |
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| Keywords: | Wheat Powdery mildew Pm21 gene FISH |
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