| 槟榔叶片总蛋白质提取及双向电泳条件初探 |
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| 引用本文： | 李涛,曾莉娟,王健华,刘志昕. 槟榔叶片总蛋白质提取及双向电泳条件初探[J]. 热带作物学报, 2009, 30(6): 746-750 |
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| 作者姓名： | 李涛 曾莉娟 王健华 刘志昕 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101
2. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101;中国热带农业科学院科技信息研究所,海南儋州,573717 |
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| 基金项目： | 国家科技支撑计划课题热带农林重要有害生物检测监测预警技术研究 |
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| 摘 要： | 采用3种提取植物组织蛋白的方法(TCA/丙酮沉淀法、E-TCA法、酚法)提取槟榔叶片总蛋白,在蛋白产量,一维和二维电泳图谱等方面对3种方法的提取效果进行比较.结果表明,TCA/丙酮沉淀法效果较好,是槟榔叶片总蛋白提取的可选方法,同时优化了双向电泳的条件和上样量.
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| 关 键 词： | 槟榔 蛋白提取 二维电泳 |
Establishment of Methods for the Extraction and Two-Dimensional Electrophoresis Analysis of Leaf Proteins of Areca catechu |
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| Li Tao,Zeng Lijuan,Wang Jianhua and Liu Zhixin. Establishment of Methods for the Extraction and Two-Dimensional Electrophoresis Analysis of Leaf Proteins of Areca catechu[J]. Chinese Journal of Tropical Crops, 2009, 30(6): 746-750 |
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| Authors: | Li Tao Zeng Lijuan Wang Jianhua Liu Zhixin |
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| Affiliation: | Li Tao1,Zeng Lijuan1,2,Wang Jianhua1,Liu Zhixin1 1 Institute of Tropical Bioscience , biotechnology,CATAS,Ministry of Agriculture Key Laboratory for Tropical Crop Biotechnology,Haikou,Hainan 571710,2 Institute of Scientific , Technological Information,Danzhou,Hainan,573717 |
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| Abstract: | Proteins from betel nut (Areca catechu Linn.)were extracted by using three methods [trichloroacetic acid (TCA) /acetone precipitation, trichloroacetic acid precipitation (E -TCA), and phenol extraction], and their productivity and profiles were assessed by means of SDS-PAGE and two-dimensional electrophoresis. Of these 3 methods the TCA/acetone precipitation method was found most effective for the extraction and two -dimensional electrophoresis of proteins from Areca leaves. The conditions of two - dimensio... |
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| Keywords: | Areca catechu Linn. protein extraction two-dimensional electrophoresis. |
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