硫酸多粘菌素E 在鸡蛋内的残留量检测及药代动力学研究

饲养20 只正处于产蛋旺期的母鸡，单笼饲养，以5 mg/kg 体重的单剂量肌注硫酸多粘菌素耘，早晚给药，2 次/d，连续注射5 d，其余正常饲喂。建立鸡蛋中硫酸多粘菌素E 的高效液相色谱-三重四级杆串联质谱（HPLCMS/MS）检测方法，从肌注第1 d开始捡蛋并检测鸡蛋内的药物残留，直至药物残留消除。以Winnonlin 药物动力学参数计算程序处理鸡蛋中药物浓度-时间数据关系，药动学主要参数为院鸡蛋中药物的达峰浓度（Cmax）为1 411.252(依578.2536) 滋g/kg，达峰时间（Tmax）为6.425844(依0.666114) d，零阶矩曲线下面积（AUC）为10 647.39(依3159.706) 滋g/kg窑d，吸收半衰期（K01-HL）为2.173256(依1.115728) d，消除半衰期（K10-HL）为2.237842(依1.072385) d，清除率（CL）为0.000503(依0.000124) L/kg窑d。

Study on preparation technology optimization of Auriculariaauricular polysaccharide by response surface method

The process of dissolving Auricularia auricular polysaccharide out effectively according to thecharacteristics of its leathery cell wall was studied. This experiment used the combination of high pressure homogenizationand enzyme method, and optimized the extraction process of polysaccharides from A. auricula by using central compositedesign method with Minitab statistical software. The results showed that, A. auricular was homogenized underhomogenization pressure 8~10 MPa for 10~12 min as broken pretreatment, then 0.05 mol/L sodium citrate buffer solutionwas used as solvent to extract for 2 h at 80. Cooling to 45, 355 U/g cellulase was added and the reaction lasted 1.2 hin the liquid ratio of 148 in pH 5.0. Then we adjusted pH to neutral with NaOH, and quickly raised the temperature to85, stayed 1 h for enzyme inactivation. Under above conditions, A. auricula polysaccharides yield reached 15.37%.