贵州苗药头花蓼rDNA ITS序列分析

以改良CTAB法提取头花蓼的基因组DNA，采用通用引物对不同来源头花蓼的rDNA ITS 序列进行PCR 扩增，测序 和聚类分析。结果表明，不同来源头花蓼有13 处变异位点，序列长度变异范围为661-666 bp，序列差异主要集中在ITS1 区与ITS2区。rDNA ITS 序列可作为分子水平鉴定头花蓼的参考依据。

Analysis of rDNA ITS sequences from Polygonum capitatum

Total genomic DNA was extracted from Polygonum capitatum by a modified cetyltrimethyl ammonium bromide (CTAB)protocol. The purified products of PCR amplifications were directly sequenced, associated with rDNA ITS sequences in triplicate, using consensus primer pairs. Nucleotide sequence analysis, phylogenetic tree and homology were performed with Clustal X software and DNAMAN programme based on DNA ITS sequences. The results indicated that the sequences of rDNA ITS were 661~666 bp in P.capitatum, and 13 variable sites, mainly concentrated in the ITS1 and ITS2 area. We argued that rDNA ITS sequences could be applied to explore molecular identification to determine the sampled eco-geographic origins in P. capitatum.