花青素合成转录因子表达载体的构建 及遗传转化油菜研究

通过 PCR 扩增得到红掌（Ant hur i um andr aeanum）的 M YB 基因全长、将 M YB 基因片段克隆到 pM D18-T 载体上、然后将此基因插入 CaM V35S启动子后构建成表达载体 Cam35S-GFP-M YB。 通过冻融法将带有目的基因的 表达载体导入根癌农杆菌、 并转化热研 2 号柱花草获得转基因植株若干。 抽取 10 株转化的柱花草植株的基因组 DNA 进行 PCR 分子验证、结果表明有 6株阳性、M YB 基因已成功整合到柱花草的基因组中。转基因柱花草植株的获 得为柱花草等豆科牧草的适口性和抗逆性的提高提供了可能、 并且为以后柱花草的遗传转化工作提供了可靠的依 据。

St udy on cons t ruct i on ofant hocyani n t rans cri pt i on f act or expres s i on vect orand t rans f orm at i on i nt o Br as s i ca napus

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