| 牛BRY基因的分子克隆及序列分析 |
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| 引用本文： | 吴冬琴,胡江,王雪郦,罗玉柱. 牛BRY基因的分子克隆及序列分析[J]. 甘肃农业大学学报, 2009, 44(3) |
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| 作者姓名： | 吴冬琴 胡江 王雪郦 罗玉柱 |
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| 作者单位： | 1. 甘肃农业大学动物科学技术学院,甘肃,兰州,730070
2. 兰州城关奶牛场,甘肃,兰州,730000 |
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| 基金项目： | 甘肃省农业生物技术研究项目，甘肃省科技攻关项目 |
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| 摘 要： | 以荷斯坦奶牛、西门塔尔牛和甘南牦牛为观测对象,利用Y染色体上特异性序列BRY基因进行性别预测,并对其进行PCR扩增、分子克隆及序列分析;最后以牛基因组DNA为模板,利用牛BRY基因作为雄性特异性基因,ZFX/ZFY作为内标基因进行双重PCR.结果表明:公牛可获得长度约为300 bp和445 bp两条扩增带的PCR产物,母牛则仅获得1条长度约为445 bp的PCR产物,与实际性别完全相符,说明该基因可用于牛的性别预测.对BRY-PCR产物克隆测序,得到300 bp的BRY基因部分核苷酸序列,与GenBank上登录的牛和绵羊的同源性序列进行比对发现,同源性都高于87.0%,表明哺乳动物的BRY基因具有高度保守性,种间差异很小.
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| 关 键 词： | 牛 BRY基因 克隆 性别预测 |
Molecular cloning and sequencing of bovine BRY gene |
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| WU Dong-Qin,HU Jiang,WANG Xue-Li,LUO Yu-zhu. Molecular cloning and sequencing of bovine BRY gene[J]. Journal of Gansu Agricultural University, 2009, 44(3) |
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| Authors: | WU Dong-Qin HU Jiang WANG Xue-Li LUO Yu-zhu |
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| Affiliation: | WU Dong-Qin1,HU Jiang1,WANG Xue-Li2,LUO Yu-zhu1(1.College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China,2.Lanzhou City Cattle Farm,Lanzhou 730000,China) |
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| Abstract: | The BRY gene was amplified,cloned and sequenced from the Holstein cattle,Simmental and yak.Cattle specific Y chromosome repetitive sequences BRY was used as a male specific gene,ZFX/ZFY gene as an internal control gene to conduct the double PCR.The results showed that under the optimized system,bull demonstrated two bands of 300 bp and 445 bp,and cow only demonstrated a band of 445 bp.The results indicated that the combined use of BRY and ZFX/ZFY loci was a highly accurate procedure for sexing bovine embryo... |
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| Keywords: | bovine BRY gene cloning sex forecast |
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