| Artificial induction of androgenetic diploid in Yellow catfish （Pelteobagrus fulvidraco） |
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| 作者姓名： | XU Hui FAN Zhaoting CHEN Weixing FANG Jingjie |
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| 作者单位： | XU Hui FAN Zhaoting~* CHEN Weixing FANG Jingjie College of Animal Science and Technology，Northeast Agricultural University，Harbin 150030，China |
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| 摘 要： | Androgenesis was the especial zoogamy that the germ plasma of offspring was from the agnate.In this study the eggs of Yellow catfish(Pelteobagrus fulvidraco)were irradiated by UV suspending in the synthetic ovarian fluid(OF),and the total dosage of UV irradiation was 220 mJ·cm~(-2).Diploid could be induced by heat shock(40℃,2 min,3 min)with different time period after fertilization(15-37 min).The result showed that heat shock with 2 min was better than 3 min;there were two apices of induction in 17-21 min and 27-31 min after fertilization.The highest hatching rate was 3.30% at 29 min after fertilization,and the difference between two apices of induction was unobvious.
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| 关 键 词： | 人工感应 黄鲶鱼 二倍体 热效应 |
| 文章编号： | 1006-8104（2007）-02-0110-05 |
| 修稿时间： | 2007-03-30 |
Artificial induction of androgenetic diploid in Yellow catfish (Pelteobagrus fulvidraco) |
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| XU Hui FAN Zhaoting CHEN Weixing FANG Jingjie.Artificial induction of androgenetic diploid in Yellow catfish (Pelteobagrus fulvidraco)[J].Journal of Northeast Agricultural University,2007,14(2):110-114. |
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| Authors: | XU Hui FAN Zhaoting CHEN Weixing FANG Jingjie |
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| Abstract: | Androgenesis was the especial zoogamy that the germ plasma of offspring was from the agnate. In this study the eggs of Yellow catfish (Pelteobagrus fulvidraco) were irradiated by UV suspending in the synthetic ovarian fluid (OF), and the total dosage of UV irradiation was 220 mJ·cm-2. Diploid could be induced by heat shock (40℃, 2 min, 3 min) with different time period after fertilization (15-37 min). The result showed that heat shock with 2 min was better than 3 min; there were two apices of induction in 17-21 min and 27-31 min after fertilization. The highest hatching rate was 3.30% at 29 min after fertilization, and the difference between two apices of induction was unobvious. |
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| Keywords: | Pelteobagrus fulvidraco heat shock androgenesis diploid |
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