人源类金属硫蛋白能促进番茄幼苗对锌的积累

金属硫蛋白具有抗辐射、抗氧化和对重金属解毒等功能,对Zn~(2+)具有很强的结合能力,在医药、农业、环境和保健等诸多领域有潜在的应用价值.将花椰菜花叶病毒35S启动子控制的人源类金属硫蛋白基因HsMT1L导入番茄基因组,结果显示,转基因番茄幼苗比野生型材料对Zn~(2+)具有更强的耐受性,并能够富集更多的Zn~(2+)离子.

Homo sapiens Metallothionein 1L (HsMT1L) Enhances the Accumulation of Zinc in Tomato Seedlings

Metallothionein (MT) has the functions of radio-resistance, oxidation-resistance and heavy-metal detoxification, and has a high binding capacity to Zn2+, and hence it has potential application value in medicine, agriculture, environment protection, health care, and others. In this study, HsMT1L, a metallothionein 1-like gene from human, which is controlled by cauliflower mosaic virus 35S promoter containing duplicated enhancer sequence (2x35S), was introduced into the genome of tomato (Solanum lycopersicum L. cv Ailsa Craig). The results showed that the tomato seedlings containing the 2x35S-HsMT1L exhibited higher Zn2+ tolerance and higher Zn2+ accumulation in the plant than the wild type.