pH值及不同百分浓度NaCl溶液对齐口裂腹鱼精子活力的影响

为研究齐口裂腹鱼的精子活力,在显微镜下观察了不同pH值及不同浓度NaCl溶液中精子的运动时间和寿命.结果表明:齐口裂腹鱼精子对pH值的适应范围在5~9之间,pH值为7时精子的活力较好,快速运动时间为(42.56±1.81)s,精子寿命为(84.33±4.77)s;齐口裂腹鱼精子最适的NaCl溶液百分浓度为0.5%,其快速运动时间为(42.78±3.03)s,寿命为(119.89±11.75)s.在较低百分浓度的NaCl溶液中,精子寿命较长,但是快速运动时间较短,当NaCl百分浓度大于0.7%时,精子基本上会被抑制,加水稀释后精子被激活且不影响其活力.pH值过低或过高都会破坏精子的生理结构,使其死亡,0.8%~0.9%NaCl溶液可用作齐口裂腹鱼精液稀释剂室温暂时保存,且短时间内不会影响其活力.

Influence of pH and Different Concentrations of NaCl Solution on Schizothorax prenanit Sperm Vitality

In order to study the sperm vitality of Schizothorax, microscopic examination was made of the movement time and life duration of the sperms of this fish species under different pH values and different NaCl concentrations. The results showed that Schizothorax sperm performed well in a pH range of 5~9, pH 7 being optimum for its vitality; its fast moving time was (42.56±1.81) s, and its life duration lasted (84.33±4.77) s, and that the optimal NaCl concentration in the solution for Schizothorax sperm was 0.5%, its fast moving time was (42.78±3.03) s, and its life duration was (119.89±11.75) s. In lower concentration of NaCl solution, the sperm lived longer, but its fast moving time was shorter. When NaCl concentration was greater than 0.7%, the sperm was largely contained. When the solution was diluted by water addition, the sperm would be re-activated and its vitality was not affected. In conclusion, pH, if too low or too high, would disrupt the physiological structure of the sperm, leading to its death; Schizothorax semen can be temporarily stored at room temperature with a 0.8%~0.9% NaCl solution as the diluent, and it will not die in a short period of time.