陆地棉衣分QTL的形态和RAPD分子标记筛选

本研究以陆地棉遗传标准系TM-1(衣分31.4%)和T586(衣分7.64%)为亲本构建衣分QTLs的作图群体.根据F2单株衣分表现,以BSA法筛选获得4个与控制陆地棉衣分QTLs连锁的RAPD分子标记.其中,OPD13947,OPAD02500,OPAO169473个标记位于同一连锁群,且与茸毛基因(T1)连锁,位于棉花的第6染色体上.该衣分QTL来自T586,标记位点可解释6.6%的表

Morphological and Molecular Tagging of Lint Percent QTLs in Upland Cotton

Four RAPD molecular markers linked to lint percent QTLs in Upland Cott on were identified using Bulked Segregant Analysis in the F 2 populations cross ed between genetic standard, TM 1(its lint percent is 31.4% ) and T586 (lint p ercen t is 7.64% ). Among them, OPD13 947 , OPAD02 500 , and OPAO16 94 7 were mapped to the s ame linkage group, the sixth chromosome, and linked to the hirsute gene T 1 . The identified QTL was supposed to from T586 and can explain the phenotypic variatio n by 6.6%. OPAK05 940 was linked to another lint percent locus contributed by TM 1 and can explain the phenotypic variation by 7.6%. Additionally, thr ee morpholo gical markers associated with variation in lint percent were identified. The all ele at T 1 increased the lint percent while the allele at brown color fiber ( L C1 ) decreased the lint percent, and can explain the phenotypic vari ation by 3.57% , and 3.85% , respectively. Naked seed ( N 1 ) had a huge negative effect on the lint percent and caused about 43.26% lint percent loss.