Levels and distribution of genetic diversity of coconut (Cocos nucifera L., var. Typica form typica) from Sri Lanka assessed by microsatellite markers |
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Authors: | L Perera JR Russell J Provan W Powell |
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Institution: | (1) Genetics and Plant Breeding Division, Coconut Research Institute, Lunuwila, Sri Lanka;(2) Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland, U.K. |
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Abstract: | The coconut variety Typica, form typica, commonly known as Sri Lanka tall coconuts is the most widely exploited and grown variety in Sri Lanka. Under the coconut
bio-diversity conservation programme, several Typica populations have been collected by island-wide surveys and planted ex situ. Thirty-three coconut populations were subjected to microsatellite assay with eight coconut-specific microsatellite primer
pairs in order to study the levels and distribution of genetic variation of the collected materials for formulating future
collection strategies and selecting parents for the breeding programme. A total of 56 alleles were detected ranging from 3
to 10 alleles per primer pair with an average of 7 alleles per locus. Overall a very high level of genetic diversity was detected
(0.999) for all the populations studied ranging from 0.526 for population Debarayaya to 0.683 for population Dickwella. Only
four introduced coconut populations, i.e. Clovis, Margeret, Dickwella, Mirishena and an embryo-cultured population were clearly
separated from the resulting dendrogram. A very high level of within population variation (99%)accounted for native populations
suggests a common history and a restricted genetic base for native Sri Lankan tall coconuts. Categorization of alleles into
different classes according to their frequency and distribution confirmed the results of the dedrogram and concluded the adequacy
of single large collection from the entire target area to represent the total genetic diversity in Sri Lanka. This study discusses
useful information regarding conservation and breeding of coconut in Sri Lanka.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | coconut Cocos nucifera conservation geneticdiversity microsatellites SSR |
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