腐胺对吸胀冷害下菜豆种子萌发及抗性的影响

为提高吸胀冷害下菜豆种子发芽率,缓解吸胀冷害对菜豆种子的伤害。以低温敏感型菜豆"热那亚"种子为试材,设置CK(常温对照)、LT(4℃低温处理)和LT+Put(4℃低温处理时加入1 mmol·L^-1的腐胺(Put))3个处理,研究外源Put对吸胀冷害下菜豆种子萌发及抗性的影响。结果表明:吸胀冷害下,外源Put处理后LT+Put组菜豆种子发芽率、发芽指数和种子活力与LT组相比分别提高了87.12%、124.21%和191.99%;LT+Put与LT处理相比细胞膜透性下降,可溶性物质含量增加,抗氧化酶活性升高,O2^-、H2O2和MDA含量增加。说明外源Put的施用可能通过降低细胞膜透性,增加可溶性物质含量,诱导抗氧化酶活性,直接或间接调节ROS清除系统来提高菜豆种子对吸胀冷害的抗性,提高菜豆种子的发芽率、发芽指数和种子活力。

Effects of Put on Seed Germination and Resistance of Phaseolus vulgaris Under Imbibition and Chilling Injury

In order to improve the germination rate of Phaseolus vulgaris seeds under imbibition and chilling injury,and alleviate the damage of imbibition and ichilling injury to snap bean seeds.In the study,the seeds of Phaseolus vulgaris ‘Renaya’,which were of low temperature sensitivity were used as experimental materials.And three treatment groups were set,respectively CK(normal temperature control),LT(4℃low temperature treatment)and LT+Put(4 ℃low temperature treatment with Put of 1 mmol·L^-1 added)to study the effect of exogenous Put on the germination and resistance of Phaseolus vulgaris seeds under imbibitional chilling.The results showed that the germination rate,germination index and seed vigor in group LT+Put were increased by 87.12%,124.21% and 191.99%,respectively,compared with the group LT.Also,as for the seeds of group LT+Put,their cell membrane permeability and soluble material content increased,antioxidant enzyme activity and content of O2^-,H2O2 and MDA also increased compared with those in group LT.It indicated that the addition of exogenous Put may increase the cell membrane permeability and the content of soluble material,induce the activity of antioxidant enzymes,directly or indirectly regulate the ROS scavenging system to improve the resistance of Phaseolus vulgaris seeds to imbibitional chilling,and improve the germination rate,germination index and seed vigor of Phaseolus vulgaris seeds.