| 红榉不同种源核rDNA的ITS序列克隆与亲缘关系 |
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| 引用本文： | 王旭军,程勇,吴际友,张玖荣,刘伟强.红榉不同种源核rDNA的ITS序列克隆与亲缘关系[J].湖南林业科技,2013(4):7-10. |
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| 作者姓名： | 王旭军 程勇 吴际友 张玖荣 刘伟强 |
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| 作者单位： | [1]湖南省林业科学院,湖南长沙410004 [2]桑植县林业局,湖南桑植427100 |
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| 基金项目： | 国家“十二五”科技支撑计划（2011BAD38803）. |
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| 摘 要： | 通过克隆和测定核糖体DNA内转录间隔区（Ribosomal DNA internal transcribed spacer,rDNA,ITS）基因序列,研究红榉不同种源间的亲缘关系。测定结果表明：红榉5个种源的ITS序列、ITS1和ITS2长度变异范围分别为561～623 bp、157～210 bp和215～222 bp。软件DNAMAN、遗传距离和UPGMA法系统发育树分析结果表明：南京种源与赣州种源的亲缘关系较近,湖州种源与怀化种源的亲缘关系较近;滁州种源单独聚为一类,与湖州、怀化种源的亲缘关系较远。
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| 关 键 词： | 红榉 种源 ITS 亲缘关系 |
Determination of nucleotide sequences of ribosomal DNA internal transcribed spacer in Zelkova schneideriana collected from different provenances |
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| WANG Xujun,CHENG Yong,WU Jiyou,ZHANG Jiurong,LIU Weiqiang.Determination of nucleotide sequences of ribosomal DNA internal transcribed spacer in Zelkova schneideriana collected from different provenances[J].Hunan Forestry Science & Technology,2013(4):7-10. |
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| Authors: | WANG Xujun CHENG Yong WU Jiyou ZHANG Jiurong LIU Weiqiang |
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| Institution: | 1. Hunan Academy of Forestry, Changsha 410004, China; 2. Forestry Bureau of Sangzhi County, Sangzhi 427100, China) |
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| Abstract: | The genetic relationship of different provenances of Zelkova schneideriana was investigated by cloning and measuring the se- quences of the nucleotide sequences of ribosomal DNA internal transcribed spacers （ITS） collected from different parts of China. The restdts showed that the length of ITS sequence, ITS 1 and ITS 2 ranged 561 -623 bp, 157 -210 bp and 215 -222 bp, respectively. And the DNAMAN, genetic and UPGMA analysis revealed that the relationships between Nanjing provenance and Ganzhou provenance, and between Huzhou provenance and Huaihua provenance were closer than the others, and the Chuzhou provenances was clustered one single group. |
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| Keywords: | Zelkova schneideriana provenance ITS genetic relationship |
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