濒危植物太行菊组织培养及快繁技术研究

研究旨在建立一种珍惜濒危植物太行菊的高效再生方法,为工厂化生产提供理论基础。以MS为基本培养基,用不同激素组合对太行菊无菌苗的叶片和茎段离体培养及植株再生、炼苗移栽等过程进行研究。结果表明,太行菊的叶片分化不定芽比茎段难,茎段最适合作为外植体进行愈伤组织的诱导及不定芽分化;筛选出了最佳培养基MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,在此培养基中,茎段外植体可一步成苗,不需转换培养基即可完成愈伤组织的诱导分化及不定芽增殖过程;最佳生根培养基为1/2MS+NAA 0.2 mg/L;再生苗在花园土中移栽成活率可达80%。研究简化了培养流程,建立了太行菊一步式高效再生体系。

Study on Tissue Culture and Rapid Propagation of the Endangered Species Opisthopappus taihangensis

The study aims to establish a high efficient regeneration system of the endangered species Opisthopappus taihangensis, and provide fundamental basis for protection and exploitation of the germplasm resource. The regeneration process from leaves and stems as explants and transplanting were studied in MS basic media with different combinations of plant growth regulators. The results showed that differentiation of callus to adventitious buds from leaves was more difficult than from stem section. Stem was the optimum explants for induction of callus and buds; the optimum medium was MS 6-BA 1.0 mg/L NAA 0.1 mg/L, in which the stem explants could regenerated by one-step method, namely, the callus was able to be reduced and differentiated to regenerated shoots, avoiding transferring to other media. The optimum rooting medium was 1/2MS NAA 0.2 mg/L. The transplanting survival rate of regenerated plantlets in garden soil reached 80%. This study simplified culture process and established a high efficient regeneration system of Opisthopappus taihangensis by one-step method.