驱蚊香草叶片组织培养研究

实现驱蚊香草的快速繁殖。利用组织培养的方法,采用激素调控技术,对驱蚊香草叶片外植体进行脱分化、再分化、丛芽增殖及其生根技术研究。在添加NAA的MS培养基上,驱赶蚊香草叶片能够形成愈伤组织,并且在愈伤组织上有根的分化;叶片在添加BA和NAA所有处理组合均有芽的分化,最适宜的分化培养基:MS BA 1.0 mg/L NAA 0.2 mg/L;丛芽增殖适宜的培养基:MS BA 0.25mg/L NAA 0.1mg/L;最佳的生根培养基:MS NAA 0.05mg/L。试管苗移栽基质以混合基质为宜。采用驱蚊香草叶片作为外植体利用组织培养的方法,可以实现驱蚊香草的快速繁殖。

Tissue culture by the leaf blade of Salvia officinalis

Objective: Rapid propagation was achieved in driving mosquito herb.Method: Applying tissue culture method and hormone regulating technique,dis-differentiation,re-differentiation,cluster shoots proliferation and theirs rooting were studied in driving mosquito herb.Result: Leaves as explant can formed callus on MS of adding NAA and differentiated roots on callus in driving mosquito herb or differentiated Shoots on MS of all combinations adding BA and NAA.The best suitable medium of shoots differentiation in leaves is MS BA 1.0 mg/L NAA 0.2 mg/L.The best suitable medium of cluster shoots proliferating is BA 0.25mg/L NAA 0.1mg/L.The best suitable rooting medium is MS NAA 0.05mg/L.The test tube seedlings were suitably transplanted in mixed matters.Conclusion: Applying leaves as explants,rapid propagation of seedlings can be achieved with plant tissue culture in driving mosquito herb.