| RAPD在油用向日葵自交系鉴定和遗传分析中的应用 |
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| 引用本文： | 王茅雁,张海明,李德军,张树军.RAPD在油用向日葵自交系鉴定和遗传分析中的应用[J].内蒙古农业大学学报(自然科学版),2001,22(2):52-55. |
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| 作者姓名： | 王茅雁 张海明 李德军 张树军 |
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| 作者单位： | 1. 内蒙古农业大学生物工程系，呼和浩特 010018
2. 中国农业大学，北京 010010 |
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| 摘 要： | 利用RAPD标记对8个油用向日葵自交系进行了鉴定和遗传分析。从40个UBC引物中筛选出12个能产生稳定遗传多态性的引物，利用其中3个引物提供的RAPD标记可将8个自交系逐一加以鉴别。12个引物对8个自交系共扩增出41条带，其中39条（占95．1％J）具有多态性，每个引物可扩增出1－7条带，平均为3．4条带，不同自交系之间的RAPD遗传距离在0．13－0．85之间，平均为0．42。按UPGMA方法可将8个自交系聚为2大类、4个亚类。
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| 关 键 词： | 油用向日葵自交系 RAPD 鉴定 遗传距离 聚类分析 |
| 文章编号： | 1009-3575(2001)02-0052-05 |
| 修稿时间： | 2001年1月15日 |
APPLICATION OF RAPD TO
IDENTIFICATION AND GENETIC ANALYSIS OF OIL SUNFLOWER INBRED LINES |
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| WANG Mao-yan,ZHANG Hai-min,LI De-jun,ZHANG Shu-jun.APPLICATION OF RAPD TO
IDENTIFICATION AND GENETIC ANALYSIS OF OIL SUNFLOWER INBRED LINES[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2001,22(2):52-55. |
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| Authors: | WANG Mao-yan ZHANG Hai-min LI De-jun ZHANG Shu-jun |
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| Abstract: | The identification and genetic analysis of 8 oil sunflower inbredlines were carried out by using RAPD markers.12 effective primers were selected from 40 arbitrary,10-mer primers,and according to the RAPD markers produced by 3 of these primers,all the inbred lines could be identified respectively.of the 12 primers tested against the 8 genotypes,a total of 41 bands were amplified,among which 39 (95.1%) were polymorphic.The products amplified by each primer ranged from1～7,with an average of 3. 4. The genetic distance between two arbitrary inbred lines ranged from 0.13～0.85,with an average of 0.42. The 8 inbred lines could be divided into 2 groups by using UPGMA cluster analysis,and each group could be divided into 2 subgroups. |
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| Keywords: | oil sunflower inbred lines RAPD identification genetic distance cluster analysis |
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