Differentiation of vaccine and field strains of bovine herpesvirus type 1 by restriction endonuclease analysis |
| |
| Authors: | C Hamelin C Jacques R Assaf |
| |
| Institution: | Centre de recherche en virologie, Institut Armand-Frappier, Laval-des-Rapides, Québec, Canada. |
| |
| Abstract: | Bovine herpesvirus type 1 (BHV-1) DNA molecules obtained from a limited number of infected cells were cleaved with a variety of restriction endonucleases. By use of selected DNA fragments in Bgl 1, Pst 1, Pvu 1 and Pvu 11 restriction patterns, one reference, two vaccine and three wild-type strains of BHV-1 were distinguished from one another. The simplified DNA fingerprinting method described here should be most useful, not only to control the genetic stability of BHV-1 vaccines during production, but also to differentiate the vaccine strains from other isolates in clinical cases. |
| |
| Keywords: | |
|
|
