稻曲病菌白化菌株生物学特性研究

 从水稻白叶枯病菌培养滤液中提取到毒素。菌体培养滤液和毒素对抗、感水稻品种的作用不一致,培养滤液中可能含有激发子。毒素稀释液抑制水稻出苗,引起水稻幼苗萎蔫和烟草坏死反应。该毒素薄层层析显示含有七种组分,野生菌与毒性基因突变体毒素组分大体相同,但含量有明显差异。不同小种间的毒素产量有区别,组分区别不大。

PATHOGEN OF WHITE FALSE SMUT OF RICE

A new strain of false smut of rice-White False Smut of Rice (WFSR) has white stromata.The chlamydospores have smooth surface with size 3.5~6.0μm×3.5~6.8μm.Culturing in malt sugar-rice but soaking liquid,WFSR can product more conidia.The optimum pH value for it's colonygrowth is pH.Several C-sources and N-sources in the experiment increased the growth of colony onsolid medium or in liqiud,and the hyphae grew best under% sucrose as C-source or 0.2% -0.3% L-Asn as N-source.WFSR produce toxins both in solid and in liquid culture,and toxins producedmore in solid culture than in liquid culture.Inoculating WFSR onto rice,it on formed white smuts.Electrophoresis for esterase-isozyme and DNA's RAPD has remarkably different bands between WF-SR and false sumt of rice,therefore,it is consided that the WFSR should be a new and different falsesmut of rice in classification status.