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丹参药材HPLC-DAD指纹图谱稳定性研究
引用本文:盛东峰,李俐俐.丹参药材HPLC-DAD指纹图谱稳定性研究[J].热带作物学报,2013,34(4):781-788.
作者姓名:盛东峰  李俐俐
作者单位:周口师范学院生命科学系,河南周口,466001
基金项目:河南省自然科学基础研究项目(No. 2010B230013)。
摘    要:指纹图谱已被认为是中药鉴定和质量控制的有效手段之一,高效液相色谱法也相应成为构建中药指纹图谱的最佳选择之一,但其稳定性一直受到质疑.本研究以丹参为材料,比较不同制备方法下,样品色谱图谱的差异,结果表明:提取温度、时间和溶剂体积微小变化对丹参指纹图谱的相似性没有影响,不同处理间指纹图谱的夹角余弦和相似性系数都在0.9以上.对流动相、检测波长、流速、柱温的研究中也得到了同样的结果.因此认为,HPLC-DAD法用于建立中药指纹图谱的稳定性和重现性好,是一种较为可靠的中药鉴定和质量控制方法.

关 键 词:高效液相色谱  指纹图谱  丹参  中药  质量控制

Stability of Salvia miltiorrhiza Bunge Fingerprints by HPLC-DAD for Quality Control
SHENG Dongfeng and LI Lili.Stability of Salvia miltiorrhiza Bunge Fingerprints by HPLC-DAD for Quality Control[J].Chinese Journal of Tropical Crops,2013,34(4):781-788.
Authors:SHENG Dongfeng and LI Lili
Institution:Department of Life Science, Zhou kou Normal University;Department of Life Science, Zhou kou Normal University
Abstract:Fingerprint is considered as a credible means for identification and quality control of traditional Chinese medicine(TCM). High performance liquid chromatography(HPLC)has been a preferable method to construct fingerprints. But its stability has been oppugned for a long time. In this study, taking Salvia miltiorrhiza Bunge as a material, chromatograms were obtained under different sample preparation methods. The results showed that small changes of extraction temperature, time and solvent volume had no influence on similarities of fingerprints(the correlation coefficient and cosine of vectorial angle of relative common peak area are all more than 0.9). The same results were obtained when different chromatographic conditions, such as mobile phase, wavelength, flow-rate and column temperature were studied. So it was concluded that HPLC fingerprints of TCM were reproducible and stable. It was a credible method for identification and quality control of TCM.
Keywords:High performance liquid chromatography  Fingerprint  Salvia miltiorrhiza Bunge  TCM  Quality control
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