| 花生单粒种子脂肪酸含量的气谱快速无损测定(英文) |
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| 引用本文： | 杨传得,关淑艳,唐月异,王秀贞,吴琪,宫清轩,王传堂. 花生单粒种子脂肪酸含量的气谱快速无损测定(英文)[J]. 花生学报, 2012, 0(3): 21-26 |
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| 作者姓名： | 杨传得 关淑艳 唐月异 王秀贞 吴琪 宫清轩 王传堂 |
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| 作者单位： | [1]山东省花生研究所,山东青岛266100 [2]吉林农业大学,吉林长春130118 |
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| 基金项目： | 国家花生产业技术体系（CARS-14）; 青岛市科技发展计划项目（09-1-3-67-jch,10-3-3-30-nsh）; 山东省科技攻关项目（2009GG10009008） |
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| 摘 要： | 描述了一种利用5～20mg花生种子组织测定花生8种脂肪酸的方法。于室温下制备用于色谱分析的样品耗时35min,气相色谱分析则需要18min。研究结果证实,本法准确性高,重复性较好,可满足花生遗传育种研究中对种子无损取样及基因聚合育种需要,将为花生脂肪酸成分的遗传改良提供便利。
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| 关 键 词： | 花生 GC 脂肪酸 测定 |
Rapid Non-destructive Determination of Fatty Acids in Single Groundnut Seeds by Gas Chromatography |
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| YANG Chuan-de,GUAN Shu-yan,TANG Yue-yi,WANG Xiu-zhen,WU Qi,GONG Qing-xuan,WANG Chuan-tang. Rapid Non-destructive Determination of Fatty Acids in Single Groundnut Seeds by Gas Chromatography[J]. Journal of Peanut Science, 2012, 0(3): 21-26 |
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| Authors: | YANG Chuan-de GUAN Shu-yan TANG Yue-yi WANG Xiu-zhen WU Qi GONG Qing-xuan WANG Chuan-tang |
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| Affiliation: | 1. Shandong Peanut Research Institute, Qingdao 266100, China; 2. Jilin Agricultural University, Changchun 130118, China) |
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| Abstract: | A protocol for analyzing 8 fatty acids using 5-20 mg groundnut seed tissue was described, which required 35 min for preparation at room temperature and 18 min for gas chromatography analy- sis. The results of analysis proved to be accurate and reproducible, irrespective of sampling amount and fatty acid content. The protocol caters the need for non-destructive sampling and gene pyramiding in genetic and breeding studies in groundnut, and may facilitate genetic improvement in fatty acid composition of the groundnut crop. |
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| Keywords: | groundnut GC fatty acid determination |
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