| 固始鸡α干扰素基因克隆与序列分析 |
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| 引用本文： | 宋凌云,崔保安,陈红英,方忠意,赵丽,管倩,吕晓丽.固始鸡α干扰素基因克隆与序列分析[J].河南农业大学学报,2007,41(2):192-195. |
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| 作者姓名： | 宋凌云 崔保安 陈红英 方忠意 赵丽 管倩 吕晓丽 |
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| 作者单位： | 河南农业大学牧医工程学院,河南,郑州,450002;河南省动物性食品安全重点实验室,河南,郑州,450002;河南省兽药监察所,河南,郑州,450002 |
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| 基金项目： | 河南省重大科技攻关项目(0223013800) |
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| 摘 要： | 参照GenBank上登陆的鸡α干扰素基因序列设计一对引物,应用PCR技术直接从固始鸡肝组织基因组DNA中扩增鸡α干扰素基因.将特异性片段克隆入pGEM-T Easy载体中,转化JM109感受态细胞,经质粒PCR鉴定及酶切鉴定筛选阳性菌株送往大连宝生物公司测序.测序结果表明,固始鸡α干扰素基因为582 bp.用DNAStar软件对克隆的固始鸡α干扰素基因与GenBank发表的其它品种鸡α干扰素基因序列进行同源性分析,核苷酸同源性在97.9%以上,氨基酸同源性在96.9%以上.
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| 关 键 词： | 固始鸡 IFN-α 基因克隆 序列分析 |
| 文章编号： | 1000-2340（2007）02-0192-04 |
| 收稿时间： | 2006-10-16 |
| 修稿时间： | 2006-10-16 |
Cloning and Sequence Analysis of IFN-Alpha in Gushi Chicken |
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| SONG Ling-yun,CUI Bao-an,CHEN Hong-ying,FANG Zhong-yi,ZHAO Li,GUAN Qian,L Xiao-li.Cloning and Sequence Analysis of IFN-Alpha in Gushi Chicken[J].Journal of Henan Agricultural University,2007,41(2):192-195. |
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| Authors: | SONG Ling-yun CUI Bao-an CHEN Hong-ying FANG Zhong-yi ZHAO Li GUAN Qian L Xiao-li |
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| Institution: | 1. Engineering College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China; 2. Henan Key Laboratory of Food Safety from Animal Products, Henan Province, Zhengzhou 450002, China ;3. Henan Institute of Veterinary Drug Control, Zhengzhou 450002, China |
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| Abstract: | Genome DNA was extracted and then used to amplificate chicken interferon-alpha gene directly with primers which were designed according to the sequences of chicken Interferon-alpha on the GenBank.Specific fragments were linked to pGEM-T Easy vector,then transformed to competent cells of JM109.By idenfication of plasmid PCR and enzyme digestion,positive strain was sent to Takara for sequencing.The sequencing results showed that we have obtained Interferon-alpha gene of Gushi chicken.The results of homology analysis with other chicken interferon-alpha gene published previously in the GenBank by DNAStar analysis revealed that there was identity of 97.9%at nucleotide homology and that of 96.9% at amino acid homology . |
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