Distinguishable staining with neutral red for GFP-marked and GFP-nonmarked Fusarium oxysporum strains simultaneously colonizing root surfaces |
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Authors: | Teruo Nonomura Hiromi Tajima Yuko Kitagawa Naoko Sekiya Kayoko Shitomi Mami Tanaka Kazuhiko Maeda Yoshinori Matsuda Hideyoshi Toyoda |
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Affiliation: | (1) Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University, Nara 631-8505, Japan, JP;(2) Experimental Farm, Kinki University, Wakayama, Japan, JP |
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Abstract: | Green fluorescent protein (GFP)-marked Fusarium oxysporum f. sp. melonis and nonmarked F. oxysporum f. sp. fragariae were stained with neutral red. The neutral red stained vacuoles of the fungi without disturbing GFP fluorescence in the cytoplasm. GFP-marked fungi showed fluorescent hyphae with dark-stained vacuoles, whereas nonmarked fungi were detected as nonfluorescent hyphae with dark-dotted vacuoles. Root colonization by these two fungi was monitored using this method. Microconidia attached similarly to the root surface and elongated vegetative hyphae. Only the pathogenic fungi invaded, causing necrosis at the inoculation site. Thus, the present method enabled us to track simultaneously the various formae speciales of F. oxysporum colonizing the root surface. Received: March 25, 2002 / Accepted: September 27, 2002 |
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Keywords: | Formae speciales of Fusarium oxysporum Neutral red staining GFP marking |
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