| 草莓镶脉病毒检测方法研究 |
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| 引用本文： | 陈晓军,王敬东,马洪爱,陈虞超,宋玉霞. 草莓镶脉病毒检测方法研究[J]. 北方园艺, 2010, 0(22): 123-125 |
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| 作者姓名： | 陈晓军 王敬东 马洪爱 陈虞超 宋玉霞 |
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| 作者单位： | 宁夏自治区农业生物技术重点实验室,宁夏,银川,750002;宁夏自治区农业生物技术重点实验室,宁夏,银川,750002;宁夏自治区农业生物技术重点实验室,宁夏,银川,750002;宁夏自治区农业生物技术重点实验室,宁夏,银川,750002;宁夏自治区农业生物技术重点实验室,宁夏,银川,750002 |
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| 基金项目： | 宁夏自然科学基金资助项目，宁夏科技攻关资助项目 |
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| 摘 要： | 以携带草莓镶脉病毒的植株为试材,运用一种新的信息生物学算法-Insignia设计特异性扩增引物,以现行技术标准为对照验证其准确性和可行性。通过RNA反转录扩增内标基因、病毒特异性序列和技术标准特异性序列,扩增产物大小分别为295、289、600 bp,与设计一致。结果表明:该算法得到的特异性引物能较好地满足草莓病毒检测的标准,从而为草莓病毒的准确、快速检测方法提供了一条新途径。
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| 关 键 词： | 草莓镶脉病毒 检测 Insignia |
Study of Detection Method on Strawberry Vein Banding Virus |
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| CHEN Xiao-jun,WANG Jing-dong,MA Hong-ai,CHEN Yu-chao,SONG Yu-xia. Study of Detection Method on Strawberry Vein Banding Virus[J]. Northern Horticulture, 2010, 0(22): 123-125 |
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| Authors: | CHEN Xiao-jun WANG Jing-dong MA Hong-ai CHEN Yu-chao SONG Yu-xia |
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| Affiliation: | CHEN Xiao-jun,WANG Jing-dong,MA Hong-ai,CHEN Yu-chao,SONG Yu-xia (Key Lab of Agricultural Biotechnology of Ningxia,Yinchuan,Ningxia 750002) |
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| Abstract: | The strawberry with strawberry vein banding virus was studied,employed a new bioinformatics arithmetic-insignia to design a specific primer and current technology stands as a control to check its veracity and feasibility,RT-PCR amplification produced internal control sequence(295 bp),virus specific sequence(289 bp) and stand specific sequence(600 bp),accorded with the length of design.The results showed that bioinformatics arithmetic meted to detection stand of strawberry virus and enlarged a new approach o... |
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| Keywords: | SVBV detection insignia |
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