| NCBI和cDNA文库中栽培花生EST-SSR分子标记的开发及其特点 |
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| 引用本文： | 王金彦,潘丽娟,杨庆利,禹山林.NCBI和cDNA文库中栽培花生EST-SSR分子标记的开发及其特点[J].分子植物育种,2009,7(4). |
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| 作者姓名： | 王金彦 潘丽娟 杨庆利 禹山林 |
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| 作者单位： | 山东省花生研究所,青岛,266100 |
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| 基金项目： | This research was supported by Modern Agro-industry Technology Research System，National High-Tech Research and Development Plan of China，National Project of Scientific and Technical Supporting Program |
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| 摘 要： | 本研究利用NCBI的GenBank数据库中公布的花生86 132条EST序列以及利用高油酸品种E12所创建的cDNA文库中的12 501条EST序列,对这些序列进行前期处理,总共获得非冗余且拼接较长的singleton 11 260条,contig 9 972条.通过MISA软件分析发现两个EST库中共包含有3 104个SSR位点,占到总共非冗余序列的11.08%.这些SSR位点被分成二核苷酸重复、三核苷酸重复、四核苷酸重复、五核苷酸重复、六核苷酸重复以及混合核苷酸重复等,其中三核苷酸重复占的比例最多,分别占到NCBI和cDNA文库的43.0%和56.8%,二核苷酸和五核苷酸重复占到所有重复位点的第二位和第三位,六核苷酸重复的比例最少.在所有重复基序中,AG/TC重复的数量最多,分别占到NCBI和cDNA文库的8.65%和13.42%.在三核苷酸重复中,CTT/GAA出现的频率最大,分别占到6.7%和13.42%.所有这些SSR基序的长度在4～51个之间.
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| 关 键 词： | 花生 开发 特点 NCBI和cDNA文库 |
Development and Characterization of EST-SSR Markers from NCBI and cDNA Library in Cultivated Peanut (Arachis hypogaea L.) |
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| Wang Jinyan,Pan Lijuan,Yang Qingli,Yu Shanlin.Development and Characterization of EST-SSR Markers from NCBI and cDNA Library in Cultivated Peanut (Arachis hypogaea L.)[J].Molecular Plant Breeding,2009,7(4). |
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| Authors: | Wang Jinyan Pan Lijuan Yang Qingli Yu Shanlin |
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| Institution: | Wang Jinyan Pan Lijuan Yang Qingli Yu Shanlin Sh,ong Peanut Research Institute,Qingdao,266100 |
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| Abstract: | 86 132 ESTs downloaded from GenBank in NCBI and 12 501 ESTs from cDNA library constructed by high-oil linoleic acid accession E12 were analysed. After the preprocession, there were 18 051 singletons and 9 972 contigs in the GenBank of NCBI and cDNA library. Totally 3 104 SSR loci had been screened by MISA software, accounting for 11.08% for these non-redundant ESTs. All SSR loci are divided into di-nucleotide, thi-nucleotide, tetra-nucleotide, penta-nucleotide, hexa-nucleotide and multi-nucleotide etc., and... |
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| Keywords: | EST-SSR Peanut (A rachis hypogaea L ) EST-SSR Development Characterization NCBI and cDNA library |
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