| 高致病性猪蓝耳病病原核酸的RT-PCR检测 |
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| 引用本文： | 王文亮,汪德生,史开志.高致病性猪蓝耳病病原核酸的RT-PCR检测[J].贵州畜牧兽医,2009,33(1):5-6. |
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| 作者姓名： | 王文亮 汪德生 史开志 |
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| 作者单位： | 1. 贵州大学动物科学学院,贵州,贵阳,550025
2. 贵州大学动物疫病研究所,贵州,贵阳,550025 |
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| 基金项目： | 贵州省农业科技攻关重大项目，贵阳市农业局科技计划项目，贵州省农业厅兽医科技计划项目 |
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| 摘 要： | 对高致病性猪蓝耳病病毒（PRRSV）Nsp2基因缺失变异特征，设计特异性引物，建立RT—PCR方法，对疑似高致病性猪蓝耳病病例进行病原核酸检测，并对扩增所得片段进行克隆测序，通过序列分析以验证RT—PCR扩增的特异性。结果表明：发病猪为高致病性猪蓝耳病病毒感染，建立的RT-PCR方法能够鉴别诊断经典猪蓝耳病和高致病性猪蓝耳病感染。
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| 关 键 词： | 高致病性猪蓝耳病 NSP2基因 RT—PCR 诊断 |
Detection of High Pathogenic Porcine Reproductive and Respiratory Syndrome Viruse RNA by RT-PCR |
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| Wang Wenliang,Wang Desheng,Shi Kaizhi.Detection of High Pathogenic Porcine Reproductive and Respiratory Syndrome Viruse RNA by RT-PCR[J].Guizhou Animal Science and Veterinary Medicine,2009,33(1):5-6. |
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| Authors: | Wang Wenliang Wang Desheng Shi Kaizhi |
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| Institution: | 1.College of Animal Science;Guizhou University;Guizhou Guiyang 550025 China;2.Institute of animal disease;Guizhou Guiyang 550025;China |
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| Abstract: | According to the Nsp2 gene deletion feature of high pathogenic PRRSV,designed specific primers and established RT-PCR method to detecte the suspect high pathogenic PRRSV RNA from infected porcines,The cloned and sequenced the specific RT-PCR product,verification the specificity of RT-PCR by sequence analysis.The resultes indicate the pigs were infected by high pathogenic PRRSV,the RT-PCR metheod is suit for the high pathogenic PRRSV RNA delection. |
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| Keywords: | RT-PCR |
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