| SD大鼠肾小管上皮细胞两种原代培养及传代方法的比较 |
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| 引用本文： | 杜胜华,唐德燊,刘华锋.SD大鼠肾小管上皮细胞两种原代培养及传代方法的比较[J].湛江医学院学报,2005,23(1):10-13. |
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| 作者姓名： | 杜胜华 唐德燊 刘华锋 |
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| 作者单位： | 广东医学院肾病研究所,广东湛江,524023;广东医学院肾病研究所,广东湛江,524023;广东医学院肾病研究所,广东湛江,524023 |
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| 基金项目： | 广东医学院博士启动基金 (2 0 0 2年 ) |
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| 摘 要： | 目的：建立较理想的大鼠肾小管上皮细胞原代培养、传代及鉴定方法。方法：采用肾小管节段贴块法及0．2％胰蛋白酶消化20min两种方法进行原代培养，以0．25％胰蛋白酶(A组)、0．125％胰蛋白酶-0．02％EDTA(B组)消化传代，利用免疫细胞化学方法鉴定细胞种类。结果：两种方法均能成功培养肾小管上皮细胞，但前者较好，小管节段贴壁早。B组成功传代(4代)并鉴定为肾小管上皮细胞，A组传代失败。结论：肾小管节段贴块、0．125％胰蛋白酶-0．02％EDTA消化是大鼠肾小管上皮细胞原代培养及传代的有效方法。
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| 关 键 词： | 肾小管上皮细胞 细胞培养 免疫细胞化学 |
| 文章编号： | 1005-4057(2005)01-0010-04 |
| 修稿时间： | 2004年3月15日 |
Comparison of primary and passage cultures for renal tubular epithelial cells in SD rat |
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| DU Sheng-hua,TANG De-shen,LIU Hua-feng.Comparison of primary and passage cultures for renal tubular epithelial cells in SD rat[J].Journal of Guangdong Medical College,2005,23(1):10-13. |
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| Authors: | DU Sheng-hua TANG De-shen LIU Hua-feng |
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| Abstract: | Objective:To establish a practical method for primary and passage cultures in rat renal cells. Methods: Both segmental sticking of renal tubule from SD rats and digestion with 0.2% trypsin for 20 minutes were used in primary culture. Digestion with 0.25% trypsin (Group A) or with 0.125% trypsin plus 0.02% EDTA (Group B) was applied in passage culture. Cell types were identified by immunocytochemistry. Results: Two methods for primary culture were successful, but the segmental sticking was better. The passage culture was only seen in Group A, and 4 generations were achieved. Conclusion: The segmental sticking of renal tubule and digestion with 0.125% trypsin plus 0.02% EDTA are eligible methods for primary and passage cultures in rat renal cells, respectively. |
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| Keywords: | renal tubular epithelial cell cell culture immunocytochemistry |
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