Effects of the preservation medium and storage duration of domestic cat ovaries on the maturational and developmental competence of oocytes in vitro |
| |
| Authors: | Takumi YOSHIDA Md Emtiaj ALAM Keisuke HANAFUSA Yasunori TSUJIMOTO Masaya TSUKAMOTO Ryoji KANEGI Toshio INABA Kikuya SUGIURA Shingo HATOYA |
| |
| Institution: | 1)Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan;2)Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi 6205, Bangladesh |
| |
| Abstract: | We examined the effectiveness of saline, Euro-Collins solution (EC), and ET-Kyoto solution (ET-K) as preservation media for the cold storage of feline ovaries. Ovaries were maintained in these media at 4°C for 24, 48, or 72 h until oocyte retrieval. The ET-K group exhibited a higher oocyte maturation rate than the saline group after 72 h of storage. Moreover, ET-K could sustain the competence of the feline oocytes to cleave after 48 h, and the morula formation rate of the ET-K group was higher than that of the other groups after 24 and 48 h. Furthermore, the ET-K group exhibited a higher blastocyst formation rate than the other groups after storage for 24 h, and only ET-K retained the developmental competence in blastocysts after 48 h of storage. In addition, regarding the cell numbers of the blastocysts, there was no significant difference among the tested groups. In conclusion, our results indicate that ET-K is a suitable preservation medium for feline ovaries. |
| |
| Keywords: | Blastocyst Feline oocyte In vitro culture In vitro maturation Preservation |
|
|
