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The isolation of intestinal microflora of farmed red seabream (Pagrus major) and black seabream (Acanthopagrus schlegeli) at larval and juvenile stages
Institution:1. Faculty of Applied Biological Science, Hiroshima University, Fukuyama, 720 Japan;2. Hiroshima Prefectural Fish Farming Center, Takehara, 729-23 Japan;1. Cancer and Translational Medicine Research Unit, University of Oulu, The Medical Research Center Oulu, Oulu University Hospital, Aapistie 5A, 90014 Oulu, Finland;2. Department of Pathology, University of Turku, Turku University Hospital, Turku, Finland;3. Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland;4. Department of Pathology, HUSLAB, Helsinki, Finland;5. Department of Oral Diagnosis, School of Dentistry, University of Campinas (UNICAMP), Av. Limeira, 901 – Bairro Areião, CEP: 13414-903 Piracicaba, São Paulo, Brazil;6. Department of Clinical and Pathology, Faculty of Medicine of Jundiai - FMJ, Jundiai, SP, Brazil;7. Laboratório Nacional de Biociências, LNBio, CNPEM, Rua Giuseppe Máximo Scolfaro, 10.000, Polo II de Alta Tecnologia de Campinas, Campinas/SP, P.O.Box 6192, CEP 13083-970 Campinas, São Paulo, Brazil;8. Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland;3. Division of Cardiology, Vancouver General Hospital, Vancouver, Canada;5. Department of Medicine, Icahn School of Medicine at Mount Sinai, Mount Sinai Beth Israel, NY, USA;1. Department of Astronautical, Electrical and Energy Engineering, Sapienza University of Rome, Via Salaria, 851, 00138 Rome, Italy;2. School of Automation, Beijing Institute of Technology, Zhongguancun South Str. No. 5, Beijing 100081, China;1. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada;2. Department of Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada;1. Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, San Juan 670, (B8000ICN) Bahía Blanca, Argentina;2. Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Piso 4, Buenos Aires 1428, Argentina;1. Department of Urology, Urological Science Institute, Yonsei University College of Medicine, Seoul, Republic of Korea;2. Department of Urology, Ewha Womans University Mokdong Hospital, Seoul, Korea
Abstract:The aerobic bacterial flora in the intestine of farmed red seabream and black seabream was investigated at their larval and juvenile stages in relation to the microflora of ambient water and diets. The total bacterial number in the intestine was 7.4×104 and 3.4×104 colony-forming units (CFU)/fish for red seabream and black seabream, respectively. Among the intestinal flora, Vibrio (ca. 45%) and Pseudomonas (30%) were the most dominant bacteria.The total bacterial counts were 3.1×104 CFU/ml for rearing water, 2.1×107 CFU/g for live diets (rotifer and brine shrimp) and 1.2×104 CFU/g for artificial diets. Among the water bacteria, Pseudomonas (22%) and Moraxella (29%) constituted dominant groups, and in the live diets Pseudomonas (48%) was predominant. The incidence of Vibrio in waters and live diets was 7% and 11%, respectively.
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