Amplified esterase genes and their relationship with other insecticide resistance mechanisms in English field populations of the aphid,Myzus persicae (Sulzer)

Myzus persicae samples were collected from populations present on a range of field crops between 1997 and 2000. A combination of biochemical, DNA-based diagnostics and bioassays was used to assess the presence of three insecticide resistance mechanisms: elevated carboxylesterase (E4 or FE4), insensitive acetylcholinesterase and insensitive sodium channels (knockdown resistance, kdr). For the carboxylesterases, both the levels of enzyme and the type of gene present (E4 or FE4) were determined. The results showed that during the time period studied there was a dramatic reduction in the proportion of aphids with very high levels of E4 and an increase in those with lower levels of FE4. There was also a slightly different E4 gene present in a limited number of samples. The change in esterase genes was accompanied by a virtual loss of the insensitive AChE variant and a maintenance of aphids with kdr. The selection pressures and other factors leading to these changes in field populations of M persicae are discussed.