| 草地藏系绵羊心脏脂肪酸结合蛋白基因第二内含子的克隆和测序 |
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| 引用本文： | 梁梓,郑玉才,王永,傅昌秀,金素钰,王金玲,於建国.草地藏系绵羊心脏脂肪酸结合蛋白基因第二内含子的克隆和测序[J].四川草原,2007(2). |
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| 作者姓名： | 梁梓 郑玉才 王永 傅昌秀 金素钰 王金玲 於建国 |
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| 作者单位： | 西南民族大学生命科学与技术学院 四川成都610041(梁梓，郑玉才，王永，金素钰，王金玲，於建国)，四川省畜禽繁育改良总站 四川成都610041(傅昌秀) |
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| 基金项目： | 四川省十五攻关项目“草地藏系绵羊的选育与提高”(02NG004-056)，国家民委重点项目“草地藏系绵羊肉用性能的改良与利用”(03N010)的一部分 |
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| 摘 要： | 采用PCR技术对草地藏系绵羊心脏脂肪酸结合蛋白基因第2内含子进行了扩增,扩增产物经克隆和测序显示,其长度为1 893bp,该基因片段与GenBank中普通绵羊和猪的H-FABP基因第2内含子分别有99%和66.4%的同源性。实验还对22只草地藏系绵羊H-FABP基因第2内含子中174 bp的序列进行了PCR-SSCP分析,结果未检测到多态性。
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| 关 键 词： | 草地藏系绵羊 心脏脂肪酸结合蛋白 单链构象多态性 |
Clone and Sequence of the Second Intron of Heart Fatty Acid Binding Protein Gene of Tibetan Sheep in Pasture |
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| LIANG Zi,ZHENG Yu-cai,WANG Yong,FU Chang-xiu,JIN Su-yu,WANG Jin-ling,YU Jian-guo.Clone and Sequence of the Second Intron of Heart Fatty Acid Binding Protein Gene of Tibetan Sheep in Pasture[J].Journal of Sichuan Grassland,2007(2). |
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| Authors: | LIANG Zi ZHENG Yu-cai WANG Yong FU Chang-xiu JIN Su-yu WANG Jin-ling YU Jian-guo |
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| Institution: | LIANG Zi1,ZHENG Yu-cai1*,WANG Yong1,FU Chang-xiu2,JIN Su-yu1,WANG Jin-ling1,YU Jian-guo1 |
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| Abstract: | The second intron of heart fatty acid binding protein(H-FABP) gene of Tibetan sheep in Pasture was amplified by PCR method.The amplified PCR product was cloned and sequenced.The results showed that the length of fragment of H-FABP was 1 893 bp and this fragment had 99% and 66.4% of homology with the second intron of H-FABP gene of common sheep and porcine in GenBbank respectively.In addition,A 174bp-fragment of the second intron of H-FABP gene was assayed in 22 DNA samples from Tibetan sheep by PCR-SSCP,but no polymorphism was observed. |
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| Keywords: | Tibetan sheep in pasture H-FABP SSCP |
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