| 抗犊牛腹泻益生菌株BN-9产芽孢条件的优化 |
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| 引用本文： | 范会兰,姜军坡,王世英,张德全,朱宝成,宗浩. 抗犊牛腹泻益生菌株BN-9产芽孢条件的优化[J]. 湖北农业科学, 2009, 48(2) |
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| 作者姓名： | 范会兰 姜军坡 王世英 张德全 朱宝成 宗浩 |
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| 作者单位： | 四川师范大学生命科学学院,成都,610068;河北农业大学生命科学学院,河北,保定,071001;河北农业大学生命科学学院,河北,保定,071001;石家庄市动物防疫监督总站,石家庄,050000;四川师范大学生命科学学院,成都,610068 |
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| 基金项目： | 石家庄市科学研究与发展计划项目 |
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| 摘 要： | 解淀粉芽孢杆菌(Bacillus amyloliquefaciens)BN-9菌株是一株能够抑制犊牛腹泻病原菌大肠杆菌(Escherichia coli)的益生菌.为提高该菌株的芽孢生成率,对其产芽孢条件进行了优化.采用单因素试验方法,选择益生菌BN-9菌株的适宜碳源、氯源、无机盐;采用正交设计,结合SPSS软件进行分析,优化出BN-9菌株的最适宜的发酵培养基组成和发酵产芽孢条件.BN-9菌株的芽孢生成率最高时,其相应的培养基组成及发酵条件如下:蔗糖为0.25%,黄豆粕粉为1.5%,MgC12为0.01%,MnSO4为0.01%,种龄12 h,接种量4%,装瓶量100 mL·250 mL-1,培养基初始pH 8.0,摇床转速200 r·min-1.37℃发酵培养48h.通过研究,确定了BN-9菌株芽孢生成率最高时的培养基组成和发酵条件;优化后,BN-9菌株的芽孢生成率提高到93.83%.相应的细菌数为2.28x108cfu·mL-1.
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| 关 键 词： | 益生菌 解淀粉芽孢杆菌 芽孢 发酵 SPSS分析 |
Optimization of the Spore Production Conditions of Strain BN-9 as Probiotics to Prevent Calf Diarrhea |
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| FAN Hui-lan,JIANG Jun-po,WANG Shi-ying,ZHANG De-quan,ZHU Bao-cheng,ZONG Hao. Optimization of the Spore Production Conditions of Strain BN-9 as Probiotics to Prevent Calf Diarrhea[J]. Hubei Agricultural Sciences, 2009, 48(2) |
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| Authors: | FAN Hui-lan JIANG Jun-po WANG Shi-ying ZHANG De-quan ZHU Bao-cheng ZONG Hao |
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| Affiliation: | 1.College of Life Science;Sichuan Normal University;Chengdu 610068;China;2.College of Life Science;Hebei Agricultural University;Baoding 071001;Hebei;3.Shijiazhuang Central Station of Inspection for Animal Epidemic Prevention;Shijiazhuang 050000;China |
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| Abstract: | Research was done to improve ratio of spore production of Bacillus amyloliquefaciens strain BN-9, which could strongly inhibit Escherichia coli, the main pathogens causing calf diarrhea. With single factor testing method, the most suitable carbon source, nitrogen source and inorganic salts to spore production of strain BN-9 were chosen. With range analysis and general linear model analysis using SPSS software to results of orthogonal designs, the most suitable fermentation medium and spore production condit... |
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| Keywords: | probiotics Bacillus amyloliquefaciens spore fermentation SPSS analysis |
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