Susceptibility assay of Haemonchus contortus to commonly used anthelmintics in Jimma, southwest Ethiopia |
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Authors: | Sileshi Belew Jemal Hussien Feyesa Regassa Kumilachew Belay Tadele Tolosa |
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Affiliation: | College of Agriculture and Veterinary Medicine, Jimma University, P.O. Box 307, Jimma, Ethiopia. |
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Abstract: | An experimental study to investigate the susceptibility pattern of Haemonchus contortus to commonly used anthelmintics (albendazole, tetramisole, ivermectin, and triclobendazole) was conducted between June and September 2009. Adult H. contortus parasites were collected from a total of 30 sheep slaughtered at Jimma municipal abattoir. The anthelmintics were assessed for egg hatch inhibition ability against H. contortus eggs. The eggs (500 eggs/ml) were incubated with different concentrations (0.25, 0.125, 0.0625, 0.03125, and 0.015625?μg/ml) at 26°C for 48?h. Egg hatching inhibition of different anthelmintics at different concentrations was recorded. The overall mean percent inhibition showed that there was a significant difference (p?0.05) among the different concentrations. Mean egg hatch inhibition of ivermectin showed the highest result (84.90?%) at 0.25?μg/ml, followed by albendazole (78.77?%), triclobendazole (76.66?%), and tetramisole (78.98?%), respectively. The overall mean percent inhibition showed a significant difference (p?0.05) among the different anthelmintics. Further evaluation of these anthelmintics was recommended. |
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