| 大叶补血草Na~+/H~+逆向转运蛋白基因NHX1的克隆及番茄的遗传转化 |
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| 引用本文： | 宋晓丽,祝建波,周玲玲. 大叶补血草Na~+/H~+逆向转运蛋白基因NHX1的克隆及番茄的遗传转化[J]. 西北农业学报, 2009, 18(6): 192-196 |
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| 作者姓名： | 宋晓丽 祝建波 周玲玲 |
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| 作者单位： | 石河子大学,农业生物技术重点实验室,新疆石河子,832000 |
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| 基金项目： | 国家转基因专项，石河子大学科学研究发展计划"自然科学与技术创新"项目 |
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| 摘 要： | 根据NCBI公布的大叶补血草NHX1基因序列设计特异性引物,经RT-PCR方法克隆得到大叶补血草液泡膜Na+/H+逆向转运蛋白基因NHX1,构建植物融合表达载体pCAMBIA1301-NHX1,重组质粒通过农杆菌介导转化番茄,经PCR和RT-PCR鉴定,获得转基因番茄植株.本研究为大叶补血草耐盐基因NHX1的进一步功能分析和应用奠定了一定的理论基础.
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| 关 键 词： | 大叶补血草 番茄 遗传转化 |
| 收稿时间： | 2009-04-03 |
| 修稿时间： | 2009-06-05 |
Cloning and Genetic Transformation of Tomato with Na+/H+ Antiporter NHX1 from Limonium gmelinii |
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| SONG Xiaoli,ZHU Jianbo and ZHOU Lingling. Cloning and Genetic Transformation of Tomato with Na+/H+ Antiporter NHX1 from Limonium gmelinii[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2009, 18(6): 192-196 |
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| Authors: | SONG Xiaoli ZHU Jianbo ZHOU Lingling |
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| Affiliation: | The Key Laboratory of Agriculture Biotechnology, Shihezi University, Shihezi Xinjiang 832000, China;The Key Laboratory of Agriculture Biotechnology, Shihezi University, Shihezi Xinjiang 832000, China;The Key Laboratory of Agriculture Biotechnology, Shihezi University, Shihezi Xinjiang 832000, China |
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| Abstract: | Specific primers were designed according to gene NHX1 of Limonium gmelinii published on NCBI. Then, the gene was cloned by means of RT-PCR. Eukaryotic expression vector pCAM-BIA1301-NHX1 had been constructed, then transformed into tomato by Agrobacterium-mediated transformation method. Transgenic tomatos were obtained. It laid a theoretical foundation for further analysis and application of Limonium gmelinii NHX1 gene from this study. |
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| Keywords: | NHX1 |
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