Structures of SAS-6 suggest its organization in centrioles |
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Authors: | van Breugel Mark Hirono Masafumi Andreeva Antonina Yanagisawa Haru-aki Yamaguchi Shoko Nakazawa Yuki Morgner Nina Petrovich Miriana Ebong Ima-Obong Robinson Carol V Johnson Christopher M Veprintsev Dmitry Zuber Benoît |
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Affiliation: | Medical Research Council-Laboratory of Molecular Biology (MRC-LMB), Hills Road, Cambridge, UK. vanbreug@mrc-lmb.cam.ac.uk |
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Abstract: | Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers. |
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