| 多花黑麦草胚性悬浮系的建立及原生质培养 |
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| 引用本文： | 葛台明 章荣德. 多花黑麦草胚性悬浮系的建立及原生质培养[J]. 华中农业大学学报, 2000, 19(4): 310-312 |
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| 作者姓名： | 葛台明 章荣德 |
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| 作者单位： | 1. 华中农业大学作物遗传改良国家重点实验室,武汉,430070;湖北民族学院园艺系,恩施,445000
2. 华中农业大学作物遗传改良国家重点实验室,武汉,430070 |
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| 基金项目： | 湖北省教委青年项目资助 |
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| 摘 要： | 以多花原麦草（Ｌｏｌｉｕｍ ｍｕｌｔｉｆｌｏｒｕｍ Ｌａｍ．）幼穗为外植诱导愈伤组织，经高有机态氮培养基改造得到易碎型胚性愈伤组织，在１／２ＡＡ培养＋１／２ＭＳＤＬ培养基中得到了生长迅速、分散的胚性悬浮系。分离原生质体经培养后得到了再生白化苗。
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| 关 键 词： | 多花黑麦草 胚性悬浮系 原生质体培养 白花苗 |
| 修稿时间： | 1999-11-17 |
Establishmentof Embryogenic Suspension and Protoplast Culture of Italian Ryegrass |
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| Ge Taiming , Zhang Rongde Qin Falan Yu Yujun Xie Yuefeng. Establishmentof Embryogenic Suspension and Protoplast Culture of Italian Ryegrass[J]. Journal of Huazhong Agricultural University, 2000, 19(4): 310-312 |
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| Authors: | Ge Taiming Zhang Rongde Qin Falan Yu Yujun Xie Yuefeng |
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| Affiliation: | Ge Taiming 1),2) Zhang Rongde 1) Qin Falan 1) Yu Yujun 1) Xie Yuefeng 1) |
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| Abstract: | Friable calli of Italian ryegrass ( Lolium multiflorum Lam.) were obtained from young inflorescence culture by subculturing the calli in organic nitrogen rich media and were used to promote the embryogenic suspension cultures. Fast growing, fine dispersed embryogenic suspensions were established in 1/2 AA 1/2 MSDL liquid medium. Protoplasts from these suspensions were cultured. Albinos were regenerated from the protoplast culture. |
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| Keywords: | Italian ryegrass embryogenic suspension culture protoplast culture albino |
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