添加咖啡因、亚牛磺酸对牛精子功能的影响

为研究获能液中添加咖啡因和亚牛磺酸对牛精子功能的影响，本研究将荷斯坦牛冻精解冻后分别添加在含不同浓度咖啡因（0、2.5、5.0、7.5 mmol/L）或亚牛磺酸（0、5、10、20、40 μmol/L）的获能处理液中，且每个处理组加入约200 μL的精液，在CO₂培养箱里经上游处理45 min，以评估咖啡因和亚牛磺酸对牛精子活力、顶体及质膜完整性的影响，进而探讨在获能液中亚牛磺酸替代咖啡因的效果。结果显示，添加2.5、5.0 mmol/L咖啡因组经上游法获能处理后的牛精子活力和顶体完整率均显著高于对照组（P<0.05），且2.5 mmol/L咖啡因组精子活力最高；添加10、20 μmol/L亚牛磺酸组经上游法获能处理后的牛精子活力、顶体完整率和质膜完整率均显著高于对照组（P<0.05），且20 μmol/L亚牛磺酸组的精子功能参数值最高；将筛选的最佳浓度2.5 mmol/L咖啡因和20 μmol/L亚牛磺酸采用同样的方法处理，发现20 μmol/L亚牛磺酸组的精子顶体完整率和质膜完整率均显著高于2.5 mmol/L咖啡因组和对照组（P<0.05）。因此，20 μmol/L亚牛磺酸可以替代2.5 mmol/L咖啡因用于体外受精体系中的精子获能处理，有助于提高精子功能参数。

Effect of Caffeine and Hypotaurine on Sperm Function of Bovine

The effect of caffeine and hypotaurine were evaluated on sperm function of bovine in this study.Frozen-thawed semen from Holstein bulls was used in sperm capacitation media with different concentrations caffeine (0,2.5,5.0 and 7.5 mmol/L) or hypotaurine (0,5,10,20 and 40 μmol/L),200 μL semen for each treatment was submitted to swim-up for 45 min in order to evaluate the effect of caffeine and hypotaurine on bovine sperm motility,acrosome integrity and plasma membrane integrity.Furthermore,the effect of hypotaurine as substitutes for caffeine in sperm capacitation media was investigated.The results showed that compared with control group,2.5 and 5.0 mmol/L caffeine resulted in greater sperm motility and the rate of acrosome integrity(P<0.05),the greatest sperm motility was observed with 2.5 mmol/L caffeine.The sperm motility,the rate of acrosome integrity and plasma membrane integrity of 10 and 20 μmol/L hypotaurine were significantly higher than those of control group (P<0.05),20 μmol/L hypotaurine was the best on sperm function parameters.The effect of 2.5 mmol/L caffeine and 20 μmol/L hypotaurine on sperm function were evaluated.Compared with 2.5 mmol/L caffeine and control group,20 μmol/L hypotaurine resulted in greater the rate of acrosome integrity and plasma membrane integrity (P<0.05).In summary,2.5 mmol/L caffeine might be replaced by 20 μmol/L hypotaurine in sperm capacitation media,it was beneficial to improve sperm function parameters.